Author(s):
Salwa Abdul Salam, Devi T, Raju A
Email(s):
salwa02salam@gmail.com
DOI:
10.52711/0974-360X.2025.00769 
Address:
Salwa Abdul Salam1*, Devi T2, Raju A3
1Research Scholar, Chettinad Academy of Pharmaceutical Sciences, Kelambakam – 603103, Tamil Nadu, India.
2Professor, Department of Pharmaceutical Chemistry, Chettinad Academy of Pharmaceutical Sciences, Kelambakam – 603103, Tamil Nadu, India.
3Professor, Department of Pharmacology, Ahalia school of Pharmacy, Ahalia campus, Kozhippara – 678557, Kerala, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 11,
Year - 2025
ABSTRACT:
Background and Aim: Vanda tessellata (Roxb.) Hook.ex. G.Don and Pluchea lanceolata (Oliver and Hiern.), generally known as “Rasna species” in India and is popular because of its multifarious characteristics in the folk and conventional system of medicine. The content of very essential bioactive phytocompounds in these plant species makes this species pharmacologically active for the therapeutic uses. Therefore, in the present HPTLC analysis of Pluchea lanceolata (PL) and Vanda tessellata (VT) using the hydromethanolic extracts of roots and leaves was performed for determining the phytochemical profiling. Methods: HPTLC study of hydromethanolic extracts of Vanda tessellata (HMEVT) and Pluchea lanceolata (HMEVT) using root and leaves were determined using CAMAG HPTLC apparatus. Results and discussion: From the HPTLC analysis the outcomes were acquired in the pattern of chromatograms, examined with the wavelength of 254 nm, 366 nm and white light, constituting many number of peaks. Phytochemical profiling of these plant species was ascertained and are showed in the tables providing the complete number of peaks, peak heights, peak area, percent area and Rf values. Conclusions: HPTLC analysis of hydromethanolic extracts of Vanda tessellata and Pluchea lanceolata using the roots and leaves parts concluded that, a wide array of phytoconstituents which perhaps for its medicinal, therapeutic activity and justifies its classical value in traditional therapeutic use.
Cite this article:
Salwa Abdul Salam, Devi T, Raju A. HPTLC Analysis of Hydromethanolic extract of Rasna Species. Research Journal Pharmacy and Technology. 2025;18(11):5337-4. doi: 10.52711/0974-360X.2025.00769
Cite(Electronic):
Salwa Abdul Salam, Devi T, Raju A. HPTLC Analysis of Hydromethanolic extract of Rasna Species. Research Journal Pharmacy and Technology. 2025;18(11):5337-4. doi: 10.52711/0974-360X.2025.00769 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-11-31
REFERENCE:
1. Abayomi Sofowora, Eyitope Ogunbodede, Adedeji Onayade. The Role and Place of Medicinal Plants in the Strategies for Disease Prevention. African Journal of Traditional, Complementary and Alternative Medicines. 2013; 10(5): 210–229. doi:10.4314/ajtcam.v10i5.2.
2. Murugan Prasathkumar, Salim Anishaa, Chenthamara Dhrisyaa, Robert Beckya. Therapeutic and pharmacological efficacy of selective Indian medicinal plants – A review. Phytomedicine Plus. 2021; 1(2): 100029. doi: 10.1016/j.phyplu.2021.100029.
3. Pooja Srivastava, Karuna Shanker. Pluchea lanceolata (Rasana): Chemical and biological potential of Rasayana herb used in traditional system of medicine. Fitoterapia. 2012; 83: 1371 –1385. https://doi.org/10.1016/j.fitote.2012.07.008.
4. Anupam Kr Sachan, Ch V Rao, Nikhil Kumar Sachan, Vineet Kumar. Phyto chemical profiling of Pluchea lanceolata wildfrom chambal valley, India. International Journal of Development Research. 2017; 7(5): 12960-12964.
5. Deepika Arya, Vidya Patni. Pharmacognostic Profile and Phytochemical Investigation of Pluchea lanceolata Oliver and Hiern. In vivo and In vitro. International Journal of Pharmaceutical Sciences Review and Research. 2013; 22(2): 157-161.
6. Chhavi Gupta, Ss Katewa. Antimicrobial testing of Vanda tessellata leaf extracts. Journal of Medicinal and Aromatic Plant Sciences. 2012; 34 (3and4): 158–162.
7. Arijit Chaudhuri, Yuvraj Singh Sarangdevot, Mangilal Choudhary, Arpit Patidar, Pahadiya Anitakumari Rambhai. Protective effect of ethanol extract of Vanda tessellata leaves on methotrexate induced hepatotoxicity and nephrotoxicity in rats. Pharmaceutical and Biosciences Journal. 2019; 7(4): 13–16. https://doi.org/10.20510/ukjpb/7/i4/185559.
8. M Anisuzzaman Chowdhury, M Masudur Rahman, Mohammed Riaz Hasan Chowdhury, M Josim Uddin, Mohammed Abu Sayeed, M Aslam Hossain. Antinociceptive and cytotoxic activities of an epiphytic medicinal orchid: Vanda tessellata Roxb. BMC Complementary and Alternative Medicine. 2014; 14: 464. https://doi:10.1186/1472-6882-14-464.
9. Ankita Jain, A K Parashar, R K Nema, T Narsinghani. High Performance Thin Layer Chromatography (HPTLC): A Modern Analytical Tool for Chemical Analysis. Current Research in Pharmaceutical Sciences. 2014; 4(1): 8-14. doi: 10.4103/2229-4708.84436.
10. Murugesan S, Bhuvaneswari, S. HPTLC fingerprint profile of methanol extract of the marine red alga Portieria hornemannii (Lyngbye) (Silva). International Journal of Advances in Pharmaceutics. 2016; 3: 61 – 65. doi: 10.7439/ijap.v5i3.3209.
11. Duraisamy Gomathi, Ganesan Ravikumar, Manokaran Kalaiselvi, Balasubramaniam Vidya, Chandrasekar Uma. HPTLC fingerprinting analysis of Evolvulus alsinoides (L.) L. Journal of Acute Medicine. 2012; 2(3):, 77-82. https://doi.org/10.1016/j.jacme.2012.08.004
12. Ravi Mundugaru, Senthilkumar Sivanesan. Pluchea lanceolata protects hippocampal neurons from endothelin-1 induced ischemic injury to ameliorate cognitive deficits. Journal of Chemical Neuroanatomy. 2018; 94: 75–85. doi: 10.1016/j.jchemneu.2018.09.002.