Author(s):
Yufri Aldi, Atika Mayyola, Dira Hefni, Afriwardi Afriwardi, Salman Umar, Aditya Alqamal Alianta
Email(s):
yufrialdi@phar.unand.ac.id
DOI:
10.52711/0974-360X.2025.00785 
Address:
Yufri Aldi1*, Atika Mayyola1, Dira Hefni2, Afriwardi Afriwardi3, Salman Umar4, Aditya Alqamal Alianta5
1Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia.
2Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Andalas, West Sumatra, Indonesia.
3Department of Physiology, Faculty of Medicine, Universitas Andalas, Padang, West Sumatra, Indonesia.
4Department of Pharmaceutical, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia.
5Department of Socio-economic, Faculty of Animal Science, Universitas Andalas, Padang, West Sumatra, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 11,
Year - 2025
ABSTRACT:
Introduction: Moringa oleifera Lam. is a plant with medicinal properties. The Indonesian community widely uses it to boost the immune system. Aim: The study aimed to determine the effect of Moringa oleifera Lam. leaf extract on NK cell activity, total leukocyte cell count, and percentage of leukocyte cells in male white mice exposed to COVID-19 virus antigen. Material and Method: 25 white male mice (Mus musculus) were used in this study. In the control group, mice were given 0.5% Na-CMC for 7 days. In the treatment group, on the first and seventh days, mice were given the COVID-19 vaccine intramuscularly. Each group was given different treatments (0.5% Na-CMC, 10 mg/kg BW, 30 mg/kg BW, and 100 mg/kg BW Moringa oleifera Lam. leaf ethanol extract) orally for 7 days. NK cell activity, total leukocyte cell count, and leukocyte cell percentage were analysed on the eighth day. The data was analysed using one-way ANOVA and Duncan's New Multiple-Range Tests (DMRT). Result: The highest NK cell activity and the total amount of leukocytes were found in those given the vaccine and Moringa oleifera Lam. extract at a dose of 100 mg/kg BW. In addition, the lowest activity was in the group that was only given 0.5% Na-CMC. The One-way ANOVA Test and DMRT result showed significant differences (p<0.05) in NK cell activity, total number of leukocytes, and percentage of leukocytes in white male mice. Total leukocyte count increased in all groups compared to the Na-CMC 0.5. On the other hand, in the percentage of total leukocyte type, there was an increase in segmented neutrophils, band neutrophils, and lymphocytes. However, no increase in monocytes and eosinophils was noted in any group. Conclusion: The administration of Moringa oleifera Lam. leaf ethanol extract can increase NK cell activity, total leukocyte count, and certain leukocyte types in white male mice exposed to COVID-19 antigens.
Cite this article:
Yufri Aldi, Atika Mayyola, Dira Hefni, Afriwardi Afriwardi, Salman Umar, Aditya Alqamal Alianta. Effect of Moringa oleifera Lam. Extract on NK Cells and Leukocytes of White Male Mice (Mus musculus) Exposed to Antigen Virus COVID-19. Research Journal Pharmacy and Technology. 2025;18(11):5447-4. doi: 10.52711/0974-360X.2025.00785
Cite(Electronic):
Yufri Aldi, Atika Mayyola, Dira Hefni, Afriwardi Afriwardi, Salman Umar, Aditya Alqamal Alianta. Effect of Moringa oleifera Lam. Extract on NK Cells and Leukocytes of White Male Mice (Mus musculus) Exposed to Antigen Virus COVID-19. Research Journal Pharmacy and Technology. 2025;18(11):5447-4. doi: 10.52711/0974-360X.2025.00785 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-11-47
REFERENCES:
1. Rambey R, Susilowati A, Rangkuti AB, Onrizal O, Desrita D, Ardi R, Hartanto A. Plant diversity, structure and composition of vegetation around Barumun Watershed, North Sumatra, Indonesia. Biodiversitas Journal of Biological Diversity. 2021; Jul 17; 22(8). https://doi.org/10.13057/biodiv/d220819
2. Setiawan A. Keanekaragaman hayati Indonesia: Masalah dan upaya konservasinya. Indonesian Journal of Conservation. 2022; 11(1): 13-21. https://doi.org/10.15294/ijc.v11i1.34532
3. Navnath NS. Moringa oleifera: A Review on Medicinal Properties and Their Commercial Applications. Asian Journal of Research in Pharmaceutical Sciences. 2024; 14(2): 125-128. https://doi.org/10.52711/2231-5659.2024.00030
4. Marhaeni LS. Daun Kelor (Moringa oleifera) sebagai Sumber Pangan Fungsional dan Antioksidan. AGRISIA-Jurnal Ilmu-Ilmu Pertanian. 2021; 13(2). Retrieved from: https://ejournal.borobudur.ac.id/index.php/3/article/view/882
5. Pachava VR, Krishnamurthy PT, Dahapal SP, Chinthamaneni PK. An updated review on “Miracle tree”: Moringa oleifera. Research Journal of Pharmacognosy and Phytochemistry (RJPP). 2018; 10(1): 101-108. https://doi.org/10.5958/0975-4385.2018.00016.X
6. Isyraqi NA, Rahmawati D, Sastyarina Y. Studi Literatur: Skrining Fitokimia dan Aktivitas Farmakologi Tanaman Kelor (Moringa oleifera Lam). In Proceeding of Mulawarman Pharmaceuticals Conferences 16 December 2020 (Vol. 12, pp. 202-210). Retrieved from: https://prosiding.farmasi.unmul.ac.id
7. Sulasmi, Khalishah AN, Mawarni B, Hidayati L, Dhiya SS. Pemanfaatan Daun Kelor (Moringa oleifera L.) Untuk Mengatasi Anemia. Jurnal Pengemas Kesehatan. 2023; Feb 24; 2(1): 18-25. https://doi.org/10.52299/jpk.v2i1
8. Masum, NHMd, Hamid K, Zulfiker AHMd, Hossain MdK, Urmi KF. In vitro Antioxidant Activities of Different parts of the Plant Moringa oleifera Lam. Research Journal of Pharmacy and Technology. 2012. 5(12): 1532-1537.
9. Shukla, P., Tiwari, A. K., and Mishra, P. Study on Moringa oleifera Lam. Syn. Seed as a Ecofriendly Coagulant and Antimicrobial agent for Water Purification. Research Journal of Science and Technology, 2016. 8(1), 45-50. https://doi.org/10.5958/2349-2988.2016.00006.1
10. Namitha R, Anjitha A. Isolation and Antimicrobial Screening of Some Phytochemicals from Moringa oleifera Lam. Research. Journal Pharmacognosy and Phytochemical. 2019; 11(2): 70-72. https://doi.org/10.5958/0975-4385.2019.00013.X
11. Devi G, Suryadevara TN, Divyabharathi, Gopinath, Velaga LRVSAR, Ponmurugan P. Efficacy of Moringa oleifera’s Therapeutic Compounds and its Antimicrobial Activity. Research Journal Pharmacy and Technology. 2020; 13(8): 3867-3872. https://doi.org/10.5958/0974-360X.2020.00684.8
12. Mali S, Bendre S, Patil S. Overview of Pharmacognostical and Pharmacological properties of Moringa oleifera. Asian Journal of Pharmacy and Technology. 2022; 12(1): 77-83. https://doi.org/10.52711/2231-5713.2022.00013
13. Aldi Y, Permatasari D, Afdalanita S, Alianta AA. Safety Evaluation of Moringa Leaves (Moringa oleifera Lam.) on Kidney Organs in Male White Rats. Research Journal of Pharmacy and Technology. 2024; 17(11):5531-5539. https://doi.org/10.52711/0974-360X.2024.00845
14. Aldi Y, Aisyah A, Abdillah R, Alianta AA. Activity of Moringa Oleifera Lam on Liver Function and Histology in White Male Rats. Pharmacognosy Journal. 2023; 15(5). http://dx.doi.org/10.5530/pj.2023.15.174
15. Husni E, Badriyya E, Putri L, Aldi Y. The effect of ethanol extract of moringa leaf (moringa oleifera lam) against the activity and capacity of phagocytosis of macrofag cells and the percentage of leukosit cells of white mice. Pharmacognosy Journal. 2021; 13(3). http://dx.doi.org/10.5530/pj.2021.13.90
16. Mehwish HM, Riaz Rajoka MS, Xiong Y, Zheng K, Xiao H, Anjin T, Liu Z, Zhu Q, He Z. Moringa oleifera–a functional food and its potential immunomodulatory effects. Food Reviews International. 2022; Oct 3; 38(7): 1533-52. https://doi.org/10.1080/87559129.2020.1825479
17. Adijaya O, Bakti AP. Peningkatan sistem imunitas tubuh dalam menghadapi Pandemi COVID-19. Jurnal Kesehatan Olahraga. 2021 Sep;9(03):51-60. Retrieved from: https://ejournal.unesa.ac.id/index.php/jurnal-kesehatan-olahraga/article/view/40767
18. Ratajczak W, Niedźwiedzka-Rystwej P, Tokarz-Deptuła B, Deptuła W. Immunological memory cells. Central European Journal of Immunology. 2018; Apr 1; 43(2): 194-203. https://doi.org/10.5114/ceji.2018.77390
19. Abel AM, Yang C, Thakar MS, Malarkannan S. Natural killer cells: development, maturation, and clinical utilisation. Frontiers in immunology. 2018; Aug 13; 9: 1869. https://doi.org/10.3389/fimmu.2018.01869
20. Fitriah M, Tambunan BA, Kahar H, Nugraha J, Aulia FA, Aryati, Yudhawati R, Sudarsono, Tinduh D, Prakoeswa CRS, Hernaningsih Y. Characteristics of Natural Killer (NK) Cell and T Lymphocyte in COVID-19 patients in Surabaya, Indonesia. Research Journal of Pharmacy and Technology. 2022; 15(5):2198-2203. https://doi.org/10.52711/0974-360X.2022.00365
21. Zafarani A, Razizadeh MH, Pashangzadeh S, Amirzargar MR, Taghavi-Farahabadi M, Mahmoudi M. Natural killer cells in COVID-19: from infection to vaccination and therapy. Future Virology. 2023; Feb; 18(3): 177-91. https://doi.org/10.2217/fvl-2022-0040
22. Gadge SS. Antiviral and Immunity-modulating Natural Herbs in the Prevention of COVID-19. Research Journal of Pharmacognosy and Phytochemistry. 2021; 13(2): 81-94. https://doi.org/10.52711/0975-4385.2021.00014
23. Aldi Y, Salman S, Badriyah E, Alianta AA, Husni E. Pembuatan Obat Tradisional Berbahan Pegagan Embun Bagi Masyarakat Di Kenagarian VII Koto Talago Kecamatan Guguk Kabupaten 50 Kota. Jurnal Hilirisasi IPTEKS. 2022; Dec 31; 5(4): 237-46. https://doi.org/10.25077/jhi.v5i4
24. Naresh BV. A Review of the 2019 Novel Coronavirus (COVID-19) Pandemic. Asian Journal Pharmaceutical Research. 2020; 10(3):233-238. https://doi.org/10.5958/2231-5691.2020.00040.4
25. Sari MK. Sosialisasi tentang pencegahan covid-19 di kalangan siswa sekolah dasar di SD Minggiran 2 Kecamatan Papar Kabupaten Kediri. Jurnal Karya Abdi Masyarakat. 2020 Jun 30;4(1):80-3. https://doi.org/10.22437/jkam.v4i1.9821
26. Manish Kumar Balai. UK Variant COVID-19. Asian Journal of Nursing Education and Research. 2021; 11(4): 601-604. https://doi.org/10.52711/2349-2996.2021.00140
27. Ahmad S, Shoaib A, Ali S, Alam S, Alam N, Ali M, Mujtaba A, Ahmad A, Ansari, S, Ali MD. Epidemiology, risk, myths, pharmacotherapeutic management and socio economic burden due to novel COVID-19: A recent update. Research Journal Pharmacy and Technology. 2020; 13(9): 4435-4442. https://doi.org/10.5958/0974-360X.2020.00784.2
28. Sarker R, Roknuzzaman ASM, Nazmunnahar, Shahriar M, Hossain MJ, Islam MR. The WHO has declared the end of pandemic phase of COVID-19: Way to come back in the normal life. Health Sci Rep. 2023; Sep 5; 6(9): e1544. https://doi.org/10.1002/hsr2.1544
29. WHO. COVID-19 Weekly Epidemiological Update. Edition 144 Published 25 May 2023. Retrieved from https://www.who.int/publications/m/item/weekly-epidemiological-update-on-covid-19---25-may-2023
30. Hammer Q, Cuapio A, Bister J, Björkström NK, Ljunggren HG. NK cells in COVID-19—from disease to vaccination. Journal of Leukocyte Biology. 2023; Nov; 114(5): 507-12. https://doi.org/10.1093/jleuko/qiad031
31. Listiani N, Susilawati Y. Review Artikel: Potensi tumbuhan sebagai imunostimulan. Farmaka. 2019; 17(2): 222-31. https://doi.org/10.24198/farmaka.v17i2.22045.g11638
32. Salsabila AA. Uji Aktivitas Ekstrak Etanol Daun Kelor (Moringa Oleifera Lam) Terhadap Aktivitas Dan Kapasitas Fagositosis Makrofag Pada Mencit Yang Divaksinasi Dengan Vaksin H5N1 (Unpublished Bachelor Theses of Universitas Perintis Indonesia “UPERTIS”).
33. Fajri M. The potential of Moringa oleifera as immune booster against COVID 19. IOP Conference Series: Earth and Environmental Science, Volume 807, Adapting Agricultural Production to Covid-19. 2021 Jul 1 (Vol. 807, No. 2, p. 022008). IOP Publishing. https://doi.org/10.1088/1755-1315/807/2/022008
34. Mutia F, Aldi Y, Dillasamola D. Uji Efek Imunomodulator Dari Ekstrak Daun Kelor (Moringa Oleifera L.) dengan Metoda Carbon Clearance terhadap Mencit Putih Jantan 2022. (Unpublished Bachelor Theses of Universitas Andalas).
35. Kemenkes RI. Farmakope Herbal Indonesia Edisi II. Jakarta: Kementrian Kesehatan Republik Indonesia or Ministry of Health of Indonesia Republic. 2017. Retrieved from: https://farmalkes.kemkes.go.id/2020/08/farmakope-herbal-indonesia-edisi-ii-tahun-2017-3/#
36. Dillasamola D, Aldi Y, Fakhri M, Diliarosta S, Biomechy OP. Immunomodulatory effect test from moringa leaf extract (Moringa oleifera L.) with carbon clearance method in male white mice. Asian Journal Pharmaceutical Clinic Research. 2018; Sep 7; 11(9): 241-5. https://doi.org/10.22159/ajpcr.2018.v11i9.26703
37. Umar S, Gusriyani S, Afriwardi A, Aldi Y. Activities of Hydrocotyle sibthorpioides Lam. Extract in Capsule on Natural Killer and CD8 Cells in Human. Tropical Journal of Natural Product Research. 2023; Aug 1; 7(8). http://www.doi.org/10.26538/tjnpr/v7i8.29
38. Aldi Y, Aria M, Erman L. Uji efek imunostimulasi ekstrak etanol herba ciplukan (Physalis angulata L.) terhadap aktivitas dan kapasitas fagositosis sel makrofag pada mencit putih betina. Scientia. 2014; 4(1): 38-42. Retrieved from https://scholar.archive.org/work/peovizlsvzhhjl2nup4xxg3dqe/access/wayback/http://www.jurnalscientia.org/index.php/scientia/article/download/78/109
39. Ghasemzadeh M, Ghasemzadeh A, Hosseini E. Exhausted NK cells and cytokine storms in COVID-19: Whether NK cell therapy could be a therapeutic choice. Human immunology. 2022; Jan 1; 83(1): 86-98. https://doi.org/10.1016/j.humimm.2021.09.004
40. Rahman H, Aldi Y, Mayanti E. Aktifitas imunomodulator dan jumlah sel leukosit dari ekstrak kulit buah naga merah (Hylocereus lemairei (Hook.) Britton and Rose) pada mencit putih jantan. Jurnal Farmasi Higea. 2017; Mar 2; 8(1): 44-58. http://dx.doi.org/10.52689/higea.v8i1.137
41. Ardina R, Rosalinda S. Morfologi eosinofil pada apusan darah tepi menggunakan pewarnaan Giemsa, Wright, dan kombinasi Wright-Giemsa. Jurnal Surya Medika (JSM). 2018; Feb 1; 3(2): 5-12. https://doi.org/10.33084/jsm.v3i2.91
42. Syofyan S, Almahdy A, Rachmaini F, Dillasamola D. The immunostimulatory effects of Peronema canescens. Jack leaves extract in Mus musculus L. using the carbon clearance method. Tropical Journal of Natural Product Research. 2024; Jun 5; 8(3): 6503-7. https://doi.org/10.26538/tjnpr/v8i3.