Author(s):
Uttam Prasad Panigrahy, Aswini Kumar Parida, Satyabrata Bhanja
Email(s):
uttampanigrahy@gmail.com
DOI:
10.52711/0974-360X.2025.00811 
Address:
Uttam Prasad Panigrahy1*, Aswini Kumar Parida2, Satyabrata Bhanja3
1Faculty of Pharmaceutical Science, Assam Down Town University, Sankar Madhab Path, Gandhi Nagar, Panikhaiti, Guwahati, Assam, India.
2Berhampur University, Berhampur - 760007, Odisha.
3RITEE College of Pharmacy, NH-6, Chhatauna, Mandir Hasaud, Raipur, Chhattisgarh, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 12,
Year - 2025
ABSTRACT:
The quantification and analytical validation of Olanzapine was developed with a stationary phase Inertsil ODS column C18 (4.6mm×250mm, 5µm) and LC1220 Agilent HPLC instrument equipped with variable wavelength detector at 270nm and Acetonitrile: Water: Triethylamine (60:40:0.1v/v/v) was used as mobile phase passed at a flow rate 1mL / min. Elution takes place at retention time of 2.143min. Validation of method were executed as stated by ICH guidelines which illustrates linearity concentration range from 10-125µg/mL amid Correlation coefficient=0.999 with Y = 47278x+63531 regression equation obtained. LOD and LOQ for Olanzapine be 0.35 µg/ml and 1.05µg/ml correspondingly. Recovery of Olanzapine was obtained 99.47-100.01%.
Cite this article:
Uttam Prasad Panigrahy, Aswini Kumar Parida, Satyabrata Bhanja. Quantification and Analytical Validation of Olanzapine by Liquid Chromatographic Method through Isocratic Separation. Research Journal Pharmacy and Technology. 2025;18(12):5621-5. doi: 10.52711/0974-360X.2025.00811
Cite(Electronic):
Uttam Prasad Panigrahy, Aswini Kumar Parida, Satyabrata Bhanja. Quantification and Analytical Validation of Olanzapine by Liquid Chromatographic Method through Isocratic Separation. Research Journal Pharmacy and Technology. 2025;18(12):5621-5. doi: 10.52711/0974-360X.2025.00811 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-12-1
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