Author(s):
Tran Thanh Long, Do Thi Phuong Linh, Dang Minh Phuc, Dang Thi Phuong Thao, Nguyen Thi My Trinh
Email(s):
ntmtrinh@hcmus.edu.vn
DOI:
10.52711/0974-360X.2025.00828 
Address:
Tran Thanh Long1, Do Thi Phuong Linh1, Dang Minh Phuc1, Dang Thi Phuong Thao1,2,3,4, Nguyen Thi My Trinh1,2*
1Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, VNUHCM-University of Science, 227 Nguyen Van Cu, Ho Chi Minh City, 700000, Viet Nam.
2Laboratory of Molecular Biotechnology, VNUHCM- University of Science, 227 Nguyen Van Cu, Ho Chi Minh City, 700000, Viet Nam.
3Laboratory of Cancer Research, VNUHCM- University of Science, 227 Nguyen Van Cu, Ho Chi Minh City, 700000, Viet Nam.
4Vietnam National University-Ho Chi Minh City, Linh Xuan Ward, Ho Chi Minh city, 700000, Viet Nam.
*Corresponding Author
Published In:
Volume - 18,
Issue - 12,
Year - 2025
ABSTRACT:
Extracts and compounds from Elephantopus mollis (EM), a relatively well-studied herbal plant of the Asteraceae family, were proved to be substantially cytotoxic toward various cancers, including cervical carcinoma. Nevertheless, the anti-metastatic ability of this plant remains unknown. Hence, in this study, we evaluated the influence of EM extracts on the migration, invasion, and adhesion of cervical cancer SiHa cells. The non-toxic thresholds of EM extracts were identified by MTT assay and microscopic observation. Wound healing assay was employed to evaluate the effect of EM extracts on cell migration. Geltrex-coated Transwell assay, and Collagen I adhesion assessment were harnessed for cancer cell invasion and extra cellular matrix attachment, respectively. The expressions of metastasis-involved genes under the influence of chosen EM extract were quantified by RT-qPCR. The sub-lethal concentrations of EM extracts were tested on SiHa cell migratory ability via wound healing assay. Among the four extracts (ethyl acetate, ethanol, water, and petroleum ether), ethyl acetate fraction (EM-EA) was chosen due to its effectiveness at very low concentrations (i.e., 5 and 10µg/mL extract reduce cell migration by 26% and 54%, respectively). Assessment of cellular invasion and adhesion indicated the significant inhibition of EM-EA on the ability to invade the basement membrane and attach to the ECM of SiHa cells, respectively. Finally, a 40% reduced expression of a crucial metastasis-involved gene (i.e., EZR) caused by EM-EA treatment suggested the underlying mechanism of this extract on the metastasis of SiHa cells. Collectively, our findings are the first to demonstrate the effects of EM extracts on the metastatic traits of cervical cancer cells and its underline mechanism.
Cite this article:
Tran Thanh Long, Do Thi Phuong Linh, Dang Minh Phuc, Dang Thi Phuong Thao, Nguyen Thi My Trinh. The effects of Elephantopus mollis Extracts on the Migration, Invasion and Adhesion of Human Cervical Cancer Cells. Research Journal Pharmacy and Technology. 2025;18(12):5739-8 doi: 10.52711/0974-360X.2025.00828
Cite(Electronic):
Tran Thanh Long, Do Thi Phuong Linh, Dang Minh Phuc, Dang Thi Phuong Thao, Nguyen Thi My Trinh. The effects of Elephantopus mollis Extracts on the Migration, Invasion and Adhesion of Human Cervical Cancer Cells. Research Journal Pharmacy and Technology. 2025;18(12):5739-8 doi: 10.52711/0974-360X.2025.00828 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-12-18
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