Author(s):
Willy Tirza Eden, Senda Kartika Rakainsa, Endah Widhihastuti, Harjono
Email(s):
willytirzaeden@mail.unnes.ac.id
DOI:
10.52711/0974-360X.2025.00863 
Address:
Willy Tirza Eden1*, Senda Kartika Rakainsa1, Endah Widhihastuti1, Harjono2
1Department of Pharmaceutical Science, Faculty of Medicine, Universitas Negeri Semarang, Gajahmungkur, Semarang 50237, Indonesia.
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Gunungpati, Semarang 50229, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 12,
Year - 2025
ABSTRACT:
Current chemotherapy approaches for cancer have many challenges, including the emergence of resistance, side effects, and even the emergence of relapse after therapy. One of the prospective chemotherapy candidates from natural materials is Opuntia elatior Mill (OpE) extract. This study aims to develop OpE extract as a candidate for chemotherapy products by producing isolates that can be used as standards in testing and standardizing natural pharmaceutical preparations. Separation was performed using vacuum liquid chromatography on the ethyl acetate fraction (EAF). Isolation was performed using preparative thin layer chromatography (P-TLC), while purity testing used TLC with three different types of mobile phases. Isolate characterization was determined by the results of IR, 1H-NMR and 13C-NMR spectra analysis. The isolate showed the highest activity in T47D breast cancer cells which were further analyzed for cell cycle and apoptosis profiles using a flow cytometer. The confirmed anticancer isolate was an Opuntiol compound that had cytotoxic activity against T47D cells with a percentage inhibition value of 20.61 ± 3.23 at a concentration of 500 µg/mL. The Opuntiol isolate is thought to inhibit the cell cycle in the G0/G1 phase. In addition, the Opuntiol isolate was able to induce apoptosis at a concentration of 175 µg/mL by 12.9%. The molecular docking results show Opuntiol bind with medium strength towards PR and ER with binding affinity of -4.51 kcal/mol and -4.59 kcal/mol respectively. Although the bonding is not very strong, the binding residues show similar results with the native ligands progesterone and estradiol towards respective target. Opuntiol further has the potential to be a marker compound as well as an active anticancer compound in pharmaceutical preparations.
Cite this article:
Willy Tirza Eden, Senda Kartika Rakainsa, Endah Widhihastuti, Harjono. Isolation, Characterization, Anticancer Activity and In Silico Profile of Secondary Metabolite from Opuntia elatior Mill.. Research Journal Pharmacy and Technology. 2025;18(12):5971-0. doi: 10.52711/0974-360X.2025.00863
Cite(Electronic):
Willy Tirza Eden, Senda Kartika Rakainsa, Endah Widhihastuti, Harjono. Isolation, Characterization, Anticancer Activity and In Silico Profile of Secondary Metabolite from Opuntia elatior Mill.. Research Journal Pharmacy and Technology. 2025;18(12):5971-0. doi: 10.52711/0974-360X.2025.00863 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-12-53
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