Author(s):
Deventhiran M, John Wyson W, Sheik Noor Mohamed M
Email(s):
msheiknoormohamed@thenewcollege.edu.in
DOI:
10.52711/0974-360X.2025.00870 
Address:
Deventhiran M1, John Wyson W2, Sheik Noor Mohamed M3*
1Assistant Professor, Department of Biotechnology, Apollo Arts and Science College, Sendrambakkam, Madhavaram, Chennai – 600052, Tamil Nadu.
2Assistant Professor, Department of Food Processing Technology, AMET University, Chennai - 603112, Tamil Nadu.
3Assistant Professor, Department of Plant Biology and Plant Biotechnology, The New College, Chennai – 600014, Tamil Nadu.
*Corresponding Author
Published In:
Volume - 18,
Issue - 12,
Year - 2025
ABSTRACT:
Sinapis alba Linn., one of the traditionally used medicinal plant was analyzed for the immunomodulatory potential using in vitro and in silico approach. In vitro immunomodulatory analysis of S. alba ethanol seed extract was evaluated using NO (Nitric Oxide) and phagocytic assay determination assay with Raw 264.7, macrophage cell line. Result showed that there was a significant induction in NO production with 40.18% at 12.5µg/ml and maximum phagocytic stimulation of 83.08% at 12.50µg/ml. Gas Chromatography Mass Spectrometry analysis of ethanol seed extract of S. alba showed presence of 13 phytocomponents. Ligands were prepared from these 13 components. Compounds passing in-silico drug-likeness were subjected to molecular docking analysis with target protein 4NKQ (Macrophage colony stimulating factor protein). Among the 13 phytocomponents, Ethyl 13-docosenoate (ethyl erucate) bound with high docking score of 142.43 at the active site 2 with five hydrogen bonds. The findings thus suggest that S. alba possess immunomodulatory potential and the compound Ethyl 13-docosenoate (ethyl erucate) could be further investigated for incorporation in pharmaceutical applications.
Cite this article:
Deventhiran M, John Wyson W, Sheik Noor Mohamed M. In vitro and In silico approach in Evaluating the Immunomodulatory potential of Ethanol extract of Sinapis alba Linn. seed. Research Journal Pharmacy and Technology. 2025;18(12):6021-7. doi: 10.52711/0974-360X.2025.00870
Cite(Electronic):
Deventhiran M, John Wyson W, Sheik Noor Mohamed M. In vitro and In silico approach in Evaluating the Immunomodulatory potential of Ethanol extract of Sinapis alba Linn. seed. Research Journal Pharmacy and Technology. 2025;18(12):6021-7. doi: 10.52711/0974-360X.2025.00870 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-12-60
REFERENCES:
1. Peter J. Delves, Seamus J. Martin, Dennis R. Burton, Ivan M. Roitt. Roitt's Essential Immunology. John Wiley and Sons. 2017
2. Bin-Hafeez B, Haque R, Parvez S, Pandey S, Sayeed I, Raisuddin S. Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice. Int Immunopharmacol. 2003; 3(2): 257-65.
3. Ogunkunle ATJ, Olopade OR. Studies on the asthma coughs plant Crinum jagus L. (Amaryllidaceae) in Nigeria. Afr J Plant Sci. 2011; 5(2): 108–114.
4. Mandal SC, and Lakshmi SM. Pharmacognosy and phytotherapy research. In: Dwivedi PK, Dwivedi SK, Kalita MC, editors. Biodiversity and Environmental Biotechnology. Jodhpur: Scientific Publishers (India). 2007: 177–224.
5. Walaa, N.A. Immunomodulatory and natural immunomodulators. J. Allergy Inflamm. 2017; 1: e101.
6. Nadkarni KR. Indian Materia Medica. 3rd ed. Mumbai: Bombay Popular Prakashan. 1954
7. Sujatha, Ravishankar, Mariajancyrani, Chandramohan. Preliminary phytochemical investigation and antimicrobial activity of S. alba. Scholars J Appl Med Sci. 2013; 1(3): 138-41.
8. Redfern J, Kinninmonth M, Burdass D and Verran J. Using soxhlet ethanol extraction to produce and test plant material (essential oils) for their antimicrobial properties. J Microbiol Biol Educ. 2014; 15(1):45-46.
9. Praveen Kumar P, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. AJBR. 2010; 4: 191-195.
10. Griess, P. Ber. Deutsch Chem. Ges. 1879; 12: 426.
11. Park BH, Fikrig SM and Smithwick EM. Infection and nitroblue-tetrazolium reductions by neutrophils: a diagnostic aid. Lancet. 1968; 292:532.
12. Eswaramoorthy R, Hailekiros H, Kedir F, Endale M. In silico Molecular Docking, DFT Analysis and ADMET Studies of Carbazole Alkaloid and Coumarins from Roots of Clausena anisata: A Potent Inhibitor for Quorum Sensing. Adv Appl Bioinform Chem. 2021; 5(14): 13-24.
13. Rao SN, Head MS, Kulkarni A, Lalonde JM. Validation studies of the site-directed docking program LibDock. J Chem Inf Model. 2007; 47: 2159-71.
14. Jaikumar K, Sheik Noor Mohamed M, John Wyson W, Deventhiran M, Babu A, Anand D, Saravanan P. In silico docking analysis of bioactive compounds from Calophyllum inophyllum L. ethanol leaf extract against epidermal growth factor receptor (EGFR) protein. Asian J Pharm Clin Res. 2017; 10(8): 214-19.
15. Mazumder PM, Shaktiprasad P, Hitesh P, Dinakar S and Paramaguru R. Evaluation of immunomodulatory activity of Glycyrhiza glabra L. roots in combination with zing. Asian Pacific Journal of Tropical Biomedicine. 2012: 15-20.
16. Ding AH, Nathan CF and Stuehr DJ. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol. 1988; 141: 2407-12.
17. Inngjerdingen KT, Debes SC, Inngjerdingen M, Hokputsa S, Harding SE and Rolstad B. Bioactive pectic polysaccharides from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal plant, isolation and partial characterization. J. Ethnopharmacol. 2005; 101: 204-214.
18. Lopes FCM, Calvo TR, Vilegas W and Carlos IZ. Inhibition of hydrogen peroxide, nitric oxide and TNF-a production in peritoneal macrophages by ethyl acetate fraction from Alchornea glandulosa. Biol. Pharm. Bull. 2005; 28: 1726-1730.
19. Rimbach G, Park YC, Guo Q, Moini H, Qureshi N, Saliou C, Takayama K, Virgili F and Packer L. Nitric oxide synthesis and TNF-α secretion in Raw 264.7 macrophages: mode of action of a fermented papaya preparation. Life Science. 2000; 67: 679-694.
20. Engwerda CR, Andrew D, Murphy M and Mynott TL. Bromelain activates murine macrophages and natural killer cells in vitro. Cell Immunol. 2001; 210: 5-10.
21. Punturee K, Wild CP and Vinitketkumneuna U. Thai medicinal plants modulate nitric oxide and tumor necrosis factor-α in J774.2 mouse macrophages. Journal of Ethnopharmacology. 2004; 95: 183-189.
22. Kim IT, Park YM, Shin KM, et al. Antiinflammatory and antinociceptive effects of the extract from Kalopanax pictus, Pueraria thunbergiana and Rhus verniciflua. J Ethnopharmacol, 2004; 94: 165-73.
23. Pacher P, Beckman JS and Liaudet, L. Nitric oxide and peroxynitrite in health and disease. Physiol. Rev. 2007; 87: 315-424.
24. Terao J. Dietary flavonoids as antioxidants. Forum Nutr. 2009; 61: 87-94.
25. Lander HM, Jacovina AT, Davis RJ and Tauras JM. Differential activation of mitogen-activated protein kinases by nitric oxide-related species. J. Biol. Chem. 1996; 272: 19705-19709.
26. Hendriks JJA, Teunissen CE, de Vries HE and Dijkstra CD. ‘Macrophages and neurodegeneration’, Brain Research Reviews, 2005; 48: 185-195.
27. Wynn TA, Chawla A and Pollard JW. Origins and hallmarks of macrophages: Development, homeostasis, and disease. Nature. 2013; 496(7446): 445-455.
28. Sudhakarn DS, Srirekha P, Devasree LD, Premsingh S and Michael RD. Immunostimulatory effects of Tinospora cordifolia Miers leaf extract in Oreochromis mossambicus. Indian. J. Exp. Biol. 2006; 44: 726-732.
29. Manosroi A, Saraphanchotiwitthaya A and Manosroi J. Effects of Pouteria cambodiana extracts on in vitro immunomodulatory activity of mouse immune system. Fitoterapia. 2006; 77: 189-193.
30. Juvekar AR and Hule AK. In vitro immune effects of Indole-3- carbinol. Journal of Pharmacy Research. 2009; 2(7): 1224-1227.