Author(s):
Sumit Kumar Singh, Shailaja Moodithaya, Usha Adiga, Desy TM, Neha Martin Honnali, Poulami Dhar
Email(s):
shailaja.moodithaya@nitte.edu.in
DOI:
10.52711/0974-360X.2025.00818 
Address:
Sumit Kumar Singh1,2, Shailaja Moodithaya1*, Usha Adiga3, Desy TM4, Neha Martin Honnali5, Poulami Dhar1
1Nitte (Deemed to be University), KS Hegde Medical Academy, Mangalore, India , Department of Physiology.
2Manipal Academy of Higher Education, Manipal, Tata Medical College, Jamshedpur, India. Department of Physiology.
3Apollo Institute of Medical Sciences & Research, Chittoor, India. Department of Biochemistry.
4Kannur Medical College, Anjarakkandy, Kerala, India. Department of Biochemistry.
5Yenepoya (Deemed to be University), Mangalore, India. CSBMM.
*Corresponding Author
Published In:
Volume - 18,
Issue - 12,
Year - 2025
ABSTRACT:
Background: The role of Vitamin D in promoting overall cellular health and preventing age-related diseases is well established. The Sirtuin1 gene regulates cellular processes like DNA repair, promotes longevity, and is recognized as one of the molecular hallmarks of biological aging. However, the potential link between Vitamin D and SIRT I is not well-established. Objectives: The primary objective is to compare the serum sirtuin 1 level among individuals with and without vitamin D deficiency. The secondary objective is to evaluate the association of SIRT1 gene polymorphism with vitamin D levels. Methods: 87 subjects with serum vitamin D deficiency and 87 with normal vitamin D levels were participated. Serum sirtuin 1 level was measured by enzyme-linked immunosorbent assay (ELISA). Genotyping was performed with the blood sample for Single Nucleotide Polymorphism (SNP)-rs3740051 of the SIRT1 gene by using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP). A comparison of variables between the two groups was performed using the Mann-Whitney U test. Spearman’s correlation test assessed the correlation between sirtuin 1 and vitamin D. The association between SIRT1 gene polymorphism and vitamin D levels was analyzed by chi-square test. Hardy-Weinberg equilibrium was calculated for the alleles. Conclusion: The findings of the study showed serum Vitamin D deficiency attributed to higher SIRT1 gene polymorphism and the down expression of the SIRT1 gene. Therefore, the study concludes that Individuals with vitamin D deficiency exhibit altered SERT1 gene expression mediated accelerated biological aging.
Cite this article:
Sumit Kumar Singh, Shailaja Moodithaya, Usha Adiga, Desy TM, Neha Martin Honnali, Poulami Dhar. Evaluation of SIRT1 as a Potential Biomarker of Aging among Middle-Aged Individuals with Altered Serum Vitamin D Status. Research Journal Pharmacy and Technology. 2025;18(12):5663-8. doi: 10.52711/0974-360X.2025.00818
Cite(Electronic):
Sumit Kumar Singh, Shailaja Moodithaya, Usha Adiga, Desy TM, Neha Martin Honnali, Poulami Dhar. Evaluation of SIRT1 as a Potential Biomarker of Aging among Middle-Aged Individuals with Altered Serum Vitamin D Status. Research Journal Pharmacy and Technology. 2025;18(12):5663-8. doi: 10.52711/0974-360X.2025.00818 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-12-8
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