ABSTRACT:
In recent years the worldwide necessity of alternative medicinal system has led to progress of traditional system of medicines. The modern scientific techniques and in depth knowledge is the today’s need.Kumariasava is one of the popular formulations constituting Aloe vera as key ingredient. The standardisation of Ayurvedic medicine assures the quality, safety and efficacy of herbal product. The study aimed to standardize developed Kumariasava formulation for chromatographic (TLC, HPTLC, HPLC) and spectral studies including UV, IR, NMR and fluorescence spectroscopy. The extracts of formulation were evaluated in various solvents amongst which chloroform extract has recorded three well-resolved fluorescent components. However, fractions (III-V) obtained amongst these extract recognised as an important parameter for standardization of KS. The spectral and chromatographic analysis of fraction V confirmed the presence of anthraquinones that is key component of aloin.Hence, aloin thus preferably utilized as a marker component for standardisation of KS. The results recorded for KS extracts were compared with the standard aloin marker compound. The overall study revealed that the developed KS formulation exhibits standards as per pharmacopeia specifications.
Cite this article:
Pranali Gaikwad, Shitalkumar Patil. Standardization of Developed Kumaryasava Formulation utilizing Physicochemical, Spectral and Chromatographic Techniques. Research Journal of Pharmacy and Technology. 2025;18(3):1176-1. doi: 10.52711/0974-360X.2025.00170
Cite(Electronic):
Pranali Gaikwad, Shitalkumar Patil. Standardization of Developed Kumaryasava Formulation utilizing Physicochemical, Spectral and Chromatographic Techniques. Research Journal of Pharmacy and Technology. 2025;18(3):1176-1. doi: 10.52711/0974-360X.2025.00170 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-3-31
REFERENCES:
1. Mishra A, Gupta A, Gupta V, et al. Asava and aristha: An ayurvedic medicine – An overview. Int J. Pharm. Bio Arch. 2010; 1(1): 24 –30.
2. General guidelines for methodologies on research and evaluation of traditional medicine. Geneva, World Health Organization, 2000(WHO/E`DM/TRM/2000.1)
3. Mukherjee PK, Harwansh RK, Bahadur S, Banerjee S, Kar A. Evidence-Based Validation of Indian Traditional Medicine: Way Forward. World J Traditional Chinese Medicine. 2017; 2(1): 48-61. doi:10.15806/j.issn.2311-8571.2015.0018.
4. Schulz V, Hänsel R, Tyler VE. Rational phytotherapy: a physician's guide to herbal medicine. Psychology Press. Edn 4. 2001. doi:10.1007/978-3-642-98093-0
5. Das C, Das D. Essential Parameters for preparation of Ayurvedic fermented products asava and arista: An overview. Res J Pharm. Tech. 2019; 12(3): 1473-80. doi: 10.5958/0974-360X.2019.00244.
6. Saha D, Paul S, Hosen S, Emran T. Role of Ayurvedic formulation in digestion. Int. Res. J. Pharm. and Pharmacol. 2012; 2(8): 187-192.
7. Bhatt I, Rawat S, Rawal S. Antioxidants in Medicinal Plants Chapter. 2013.
8. Manmode R et al. Effect of preparation method on antioxidant activity of ayurvedic formulation Kumariasava. J Homeop Ayurv Med. 2012; 1(1):4. doi:10.4172/2167-1206.
9. Das C, Das D. An overview on therapeutic potential of traditional fermented Biomedicines: Asava and Arista.Research J Pharm. and Tech. 2019; 12(10): 5067-75. doi: 10.5958/0974-360X.2019.00879.5
10. Kokate C, Purohit A, Gokhale S, Pharmacognosy, twentyfirst ed., NiraliPrakashan. 2002; 108–109.
11. Neeli R, Kamta P, Kumar P. Standardization strategies for herbal drugs: An overview. Research J Pharm. and Tech. 2008; 1(4): 310-12.
12. Pharmacoepical standards for ayurvedic formulations, Central council for research in ayurvedic and siddha, Ministry of Health and Family Welfare, 1987, p. 21.
13. Gharate M, Patil R, Kasture V, Patil R. Evaluation of quantitative parameters of ayurvedic formulation: Kankasava. Int J of Pharm Pharm Sci. 2011; 3(1): 43-45.
14. Saonere J et al. Pharmacognostic evaluation and in-vitro evaluation of antioxidant activity of Mesuaferrea Linn. Research J Pharm. and Tech. 2023; 16(9): 4234-38.doi: 10.52711/0974-360X.2023.00693
15. Patel R. A critical review of literature on Aloe vera, Thesis Submitted to North Gujarat University.
16. Makchuchit S, Itharat A, Tewtrakul S. Antioxidant and nitric oxide inhibition activities of Thai medicinal plants. J Med Assoc Thai. 2010; 93Suppl 7:S227-S235.
17. Manzocco L, Anese M, Nicoli MC. Antioxidant properties of tea extracts as affected by processing. LWT-Food Science and Technology. 1998; 31(7-8): 694-8. https://doi.org/10.1006/fstl.1998.0491
18. Merghem M, Dahamna S, Khennouf S. In vivoantioxidant activity of RutamontanaL. extracts. J. Mater. Environ. Sci. 2019; 10(5): 470-77. doi:10.22270/jddt.v10i2.3919
19. Bhoopat L, Srichairatanakool S, Kanjanapothi D, Taesotikul T et al. Hepatoprotective effects of lychee (Litchi chinensisSonn): A combination of antioxidant and anti-apoptotic activities. J Ethnopharmacol. 2011; 136 (1): 55–66. doi: 10.1016/j.jep.2011.03.061
20. Elamthuruthya A, Shah C, Khan T, Tatkeb P,Gabhe Y. Standardization of marketed Kumariasava—An ayurvedicAloe veraproduct. J Pharm Biomed Anal. 2005; 37(5): 937–941.doi: 10.1016/j.jpba.2004.11.044
21. Jayachitra J, Janani B, Bharati V, Manikandan R. Phytochemical analysis and mineral composition of methanolic extract of Boerhaviadiffusa L. Reseach J. Pharm. and Tech. 2020; 13(10): 4856-60. doi: 10.5958/0974-360X.2020.00854.9.