Author(s):
Surekha Ramachandran, Aishwarya Vedagiri, Sumathi Thangarajan
Email(s):
rsurekha2013@gmail.com
DOI:
10.52711/0974-360X.2025.00433 
Address:
Surekha Ramachandran1*, Aishwarya Vedagiri2, Sumathi Thangarajan3
1Department of Biochemistry, SRM Dental College, Bharathi Salai, Ramapuram, Chennai – 600089, Tamil Nadu, India.
2Scientific Writer, MR International Technophile Pvt. Ltd., Tamil Nadu, India.
3Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai – 600113, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 7,
Year - 2025
ABSTRACT:
Alzheimer's disease (AD) is a multifaceted neurodegenerative condition attributed by oxidative insults and cognitive deterioration. The present research explored the possible therapeutic benefits of an ethanolic extract of Portulaca oleracea on colchinine induced oxidative damage and cognitive impairment in rats. AD was produced experimentally by intracerebroventricular (ICV) injection of Colchicine (15µg/5µL ACSF). In the treatment group, rats were pretreated with POEE (0.5 and 2mg/kg b.w. respectively) for 7 days prior to the administration of ICV colchicine (15µg/5µL ACSF on day 7) followed by continued treatment with POEE for 14 days. Microtuble disrupting chemical colchicine when administered via ICV in rats resulted in memory retention defect in behavioral paradigms and causes oxidative injury indicated by a defective antioxidant enzyme function and increased lipid peroxidation. Administration of POEE at doses 0.5 and 2mg/kg b.w. reversed memory retention potential in rats by regulating the antioxidant defense mechanism against oxidative insults in colchicine induced animals in a dose-dependent manner. Our data suggest that Portulaca oleracea could be a potential therapeutic approach for the treatment of cognitive impairment and oxidative stress associated with Alzheimer's disease.
Cite this article:
Surekha Ramachandran, Aishwarya Vedagiri, Sumathi Thangarajan. Portulaca oleracea Ethanolic Extract Ameliorates Colchicine-induced Cognitive Deficit and Oxidative Injury in Rats: A Potential Therapeutic Approach for Alzheimer’s Disease. Research Journal of Pharmacy and Technology. 2025;18(7):3024-0. doi: 10.52711/0974-360X.2025.00433
Cite(Electronic):
Surekha Ramachandran, Aishwarya Vedagiri, Sumathi Thangarajan. Portulaca oleracea Ethanolic Extract Ameliorates Colchicine-induced Cognitive Deficit and Oxidative Injury in Rats: A Potential Therapeutic Approach for Alzheimer’s Disease. Research Journal of Pharmacy and Technology. 2025;18(7):3024-0. doi: 10.52711/0974-360X.2025.00433 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-7-13
REFERENCES:
1. Choudhury S, Vellapandian C. Alzheimer’s Disease Pathophysiology and its Implications. Research Journal of Pharmacy and Technology. 2019; 12(4): 2045-2048. doi: 10.5958/0974-360X.2019.00338.X
2. Pal T, Das M. Review of Alzheimer’s Disease’s Animal Model with it’s Pathophysiology and Drug Discovery. Asian Journal of Research in Pharmaceutical Sciences. 2024; 14(1): 34-2 doi: 10.52711/2231-5659.2024.00006
3. Bhardwaj JS, Manandhar S, Chatterjee S, Hari G, Priya K, Raviteja S, Ranganath Pai K.S. Protective Effects of Gossypin in Colchicine-induced Cognitive Dysfunction and Oxidative Damage in Rats. Research Journal of Pharmacy and Technology. 2020; 13(11): 5189-5196. doi: 10.5958/0974-360X.2020.00907.5
4. Kumar MHV, Gupta YK. Intracerebroventricular administration of colchicine produces cognitive impairment associated with oxidative stress in rats. Pharmacology, Biochemistry, and Behavior. 2002; 73: 565–571.
5. Dweck AC. Purslane (Portulaca oleracea) – the global panacea. Personal Care Magazine. 2001; 2: 7-15.
6. Samy J, Sugumaran M, Lee KLW. Herbs of Malaysia: An Introduction to the medicinal, culinary, aromatic and cosmetic use of herbs. Times Edition, Kuala Lumpur 2004.
7. Simopoulos AP. Omega-3 fatty acids and antioxidants in edible wild plants. Biological Research. 2004; 37: 263-277.
8. Mukhtar HM, Mir PA, Ali B, Yadav S. Preliminary Phytochemical Standardisation of Portulaca oleracea Linn. Research Journal of Pharmacy and Technology. 2012; 5(3): 411-414.
9. Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with cancer. Life Sciences. 2004; 74: 2157-2184.
10. Radhakrishnan R, Zakaria MNM, Islam MW, Chen HB, Kamil M, Chan K, Al-Attas A. Neuropharmacological actions of Portulaca oleraceae L v. sativa (Hawk). Journal of Ethnopharmacology. 2001; 76: 171-176.
11. Hu LF, Xu XY, Wang BQ. Research and utilization situation of Portulaca oleracea L. in China. Prac J Med Pharm. 2003; 20: 315–316.
12. Jaiswal S, Rajwade D. A Review on Portulaca oleracea (Nonia bhaji): A wonderful weed of Chhattisgarh. Research Journal of Pharmacy and Technology. 2017; 10(7): 2415-2420. doi: 10.5958/0974-360X.2017.00426.7
13. Rashed AN, Afifi FU, Disi AM. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. Journal of Ethnopharmacology. 2003; 88: 131–6.
14. Zhang XJ, Ji YB, Qu ZY, Xia JC, Wang L. Experimental studies on antibiotic functions of Portulaca oleracea L. in vitro. Chinese Journal of Microecology. 2002; 14: 277–80.
15. Gong F, Li F, Zhang L, Li J, Zhang Z, Wang G. Hypoglycemic effects of crude polysaccharide from purslane. International Journal of Molecular Sciences. 2009; 10: 880-888.
16. Moneim AEA, Dkhil MA, Al-Quraishy S. The potential role of Portulaca oleracea as a neuroprotective agent in rotenone-induced neurotoxicity and apoptosis in the brain of rats. Pesticide Biochemistry and Physiology. 2013; 105: 203-212.
17. Yamana S, Tamilanban T, Chitra V. Medicinal plants Targeting Alzheimer’s disease - A Review. Research Journal of Pharmacy and Technology. 2020; 13(7): 3454-3458.
18. Pellegrino LJ, Cushman AJ. A Stereotaxic Atlas of the Rat Brain. Appleton Century Crafts, New York, 1967.
19. Jonathan AO, Etan JM. Age-related deficits on the radial maze and in fear conditioning: Hippocampal processing and consolidation. Hippocampus. 1998; 8: 402-415.
20. Lister RG. Ethologically - based animal models of anxiety disorders. Pharmacology and Therapeutics. 1990; 46: 321-340.
21. Porsolt RD, Bertin A, Jalfre M. Behavioral despair in mice: A primary screening test for antidepressants. Archives internationales de pharmacodynamie et de thérapie. 1977; 229: 327-36.
22. Jalaiah M, Sharma J, Sharma P. Krill Oil attenuates doxorubicin induced cognitive impairment by inhibiting oxidative stress markers in the brains of wistar rats. Research Journal of Pharmacy and Technology. 2023; 16(2): 652-8.
23. Utley HC, Bernheim F, Hochslein P. Effect of sulfhydryl reagent on peroxidation in microsome. Archives of Biochemistry and Biophysics. 1967; 260: 521-531.
24. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 1974; 47: 469-474.
25. Aebi H. Catalase in vitro. Methods in Enzymology. 1984;105:121-126.
26. Ellman GL. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics. 1959; 82: 70-77.
27. Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. Journal of Biological Chemistry. 1975; 250: 5475-5480.
28. Wheeler CR, Salzman JA, Elsayed NM, Omaye ST, Korte DWJ. Automated assays for superoxide dismutase,catalase, glutathione peroxidase and glutathione reductase activity. Analytical Biochemistry. 1990; 184: 193-199.
29. Habig WH, Jakoby WB. Assays for differentiation of glutathione-S-transferases. Methods in Enzymology. 1981; 77: 398-405.
30. Ellmann GL, Courtney KD, Andres VJ, Feather-stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology. 1961; 7: 88-95.
31. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the follin phenol reagent. Journal of Biological Chemistry. 1951; 193: 265-275.
32. Nakayama T, Sawada T. Involvement of microtubule integrity in memory impairment caused by colchicine. Pharmacology, Biochemistry, and Behavior. 2002; 71: 119-138.
33. Kumar VMH, Gupta YK. Intracerebroventricular administration of colchicine produces cognitive impairment associated with oxidative stress in rats. Pharmacology, Biochemistry, and Behavior. 2002; 73: 565-571.
34. Emerich DF, Walsh TJ. Cholinergic loss and cognitive impairment following intraventricular or intradentate injections of colchicine. Brain Research. 1990; 517: 157-167.
35. Reddy R, Rajesham VV, Kumar SS, Ramesh M, Suba V. Neuropharmacological profile of Portulaca olaracea L. sativa on animal models. International Journal of Experimental Pharmacology. 2011; 1: 33-36.
36. Pragati K, Kamal K, Sharm D.K. Catalase and Superoxide Dismutase (SOD) activity in Swiss Albino mice treated with Ethanolic leaf extract of Bauhinia variegata. Research Journal of Pharmacy and Technology. 2019; 12(9): 4259-4262.
37. Liochev SI, Fridovich I. Mutant Cu, Zn superoxide dismutases and familial amyotrophic lateral sclerosis: Evaluation of oxidative hypotheses. Free Radical Biology and Medicine. 2003; 34: 1383-1389.
38. Nagaraju B, Chandrasekhar K, Uma Sankar G, Koteswarao GSN, Umasankar K, Ankarao A. Neuroprotective effect of Conessinin on Elevated oxidative stress induced Alzheimers’disease in rats. Research Journal of Pharmacy and Technology. 2020; 13(6): 2703-2707.
39. Chelikani P, Fita I, Loewen PC. Diversity of structures and properties among catalases. Cellular and Molecular Life Sciences. 2004; 61: 192-208.
40. Bains JS, Shaw CA. Neurodegenerative disorders in humans: The role of glutathione in oxidative stress-mediated neuronal death. Brain Research Reviews. 1997; 25: 335-358.
41. Dkhil MA, Moniem AEA, Al-Quraishy S, Saleh RA. Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action. Journal of Medicinal Plant Research. 2011; 5: 1589-1563.
42. Yadav RSP, Shenoy BV, Kumar N, Prasanna Kumar G, Naveen Kumar S. In vivo Acetylcholinesterase activity and Antioxidant property of Cucurbita pepo ethanolic extract in Alzheimer’s disease induced by Aluminium chloride in Sprague Dawley rat model. Research Journal of Pharmacy and Technology 2023; 16(3): 1065-1. doi: 10.52711/0974-360X.2023.00178
43. Moneim AEA, Nasr IA, Dkhil MA, Al-Quraishy S. Neuronal activities of Portulaca oleracea in adult rats. Journal of Medicinal Plant Research. 2012; 6: 3162-3168.
44. Shen YX, Xu SY, Wei W, Sun XX, Yang J, Liu LH, Dong C. Melatonin reduces memory changes and neural oxidative damage in mice treated with D-galactose. Journal of Pineal Research. 2002; 32: 173-178.