Author(s):
Ramprasath C, Suganthi M, Ashok Kumar K, Jayanthi M, Amudha P, Vivek P, Sathya Priya R, Abirami G
Email(s):
drabirami.cas@gmail.com
DOI:
10.52711/0974-360X.2025.00436 
Address:
Ramprasath C3, Suganthi M1, Ashok Kumar K1, Jayanthi M1, Amudha P2, Vivek P4, Sathya Priya R1, Abirami G1*
1Department of Biotechnology, School of Life Science, Vels institute of science technology and Advanced Studies, Chennai.
2Department of Biochemistry, School of Life Science, Vels institute of science technology and Advanced Studies, Chennai.
3Eukpro Biotech Private limited, Chrompet, Chennai - 600 044, Tamil Nadu, India.
4Department of Bioengineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Chennai.
*Corresponding Author
Published In:
Volume - 18,
Issue - 7,
Year - 2025
ABSTRACT:
The Halophilic bacterium EBPL0007 was obtained from the Eukpro Biotech Private Limited, R&D Lab, Chrompet, Chennai. The Halophilic bacterium screened for four industrially important enzymes, out of which protease showed the maximum zone of clearance. Halophilic bacterium EBPL0007 was identified as Bacillus Licheniformis by polyphasic taxonomical approach and submitted in GenBank with the accession number MW387224. The medium for protease production was optimized as Glucose as a carbon source, peptone as a nitrogen source, 0.05% of gelatin as a substrate, 3% NaCl, pH-9 and 36°C, Bacillus Licheniformis was mass cultured in optimized production medium, harvested after 9 days of incubation and the enzyme protease was partially purified by Acetone precipitation method. The partially purified enzyme was lyophilized and the effect of temperature, pH, NaCl and metal ions was analyzed. The enzyme activity was found to be 3150 units/ml in partially purified. The crude enzyme was used for the washing test to compare the results with the available detergents in the market as its application.
Cite this article:
Ramprasath C, Suganthi M, Ashok Kumar K, Jayanthi M, Amudha P, Vivek P, Sathya Priya R, Abirami G. Optimization, Production and Partial Purification of an Extracellular Alkaline Protease from Halophilic Bacterium Bacillus licheniformis EBPL0007. Research Journal of Pharmacy and Technology. 2025;18(7):3045-0. doi: 10.52711/0974-360X.2025.00436
Cite(Electronic):
Ramprasath C, Suganthi M, Ashok Kumar K, Jayanthi M, Amudha P, Vivek P, Sathya Priya R, Abirami G. Optimization, Production and Partial Purification of an Extracellular Alkaline Protease from Halophilic Bacterium Bacillus licheniformis EBPL0007. Research Journal of Pharmacy and Technology. 2025;18(7):3045-0. doi: 10.52711/0974-360X.2025.00436 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-7-16
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