Author(s):
Fitry Tafzi, Surhaini, Dian Wulan Sari, Indra Lasmana Tarigan, Madyawati Latief, Sofia Nurjannah
Email(s):
fitrytafzi@unja.ac.id
DOI:
10.52711/0974-360X.2025.00551 
Address:
Fitry Tafzi1*, Surhaini2, Dian Wulan Sari3, Indra Lasmana Tarigan4, Madyawati Latief5, Sofia Nurjannah6
1,2,3Department of Agricultural Product Technology, Faculty of Agriculture, Universitas Jambi, Indonesia.
4,5,6Department of Chemistry, Faculty of Science and Technology, Universitas Jambi, Indonesia.
4,5Natural Product and Bioactice Compound Laboratory, Faculty of Science and Technology, Universitas Jambi, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 8,
Year - 2025
ABSTRACT:
Lansium domesticum (L. domesticum) is a tropical fruit widely cultivated in Indonesia, with its leaves and bark known to contain bioactive compounds. However, their biological activities remain largely unexplored. This study aimed to evaluate the antioxidant activity and cytotoxic potential of ethanol extracts from L. domesticum leaves and bark against breast cancer (MCF-7) and cervical cancer (HeLa) cells in vitro. Antioxidant activity was assessed using the DPPH radical scavenging assay, while cytotoxicity was determined using the MTT assay. The results revealed that the leaf extract exhibited stronger antioxidant activity than the bark extract. Cytotoxicity analysis demonstrated that both extracts were toxic to MCF-7 and HeLa cells, with the bark extract exhibiting higher potency. The IC50 values for the leaf extract were 71.77 µg/mL against MCF-7 cells and 37.45 µg/mL against HeLa cells, while the bark extract showed IC50 values of 58.65 µg/mL and 17.54 µg/mL against MCF-7 and HeLa cells, respectively. Furthermore, molecular docking analysis revealed that allo-aromadendrene exhibited the strongest binding affinity to both the MCF-7 (3ERT) and HeLa (5DXU) receptors, with the lowest Gibbs free energy values and inhibition constant. ?-muurolene also demonstrated a notable interaction with the HeLa receptor. These findings suggest that L. domesticum bark extract, particularly due to the presence of allo-aromadendrene and ?-muurolene, has significant cytotoxic potential and could serve as a promising candidate for the development of natural anticancer agents, particularly against cervical cancer. Further studies are warranted to explore its mechanisms of action and potential therapeutic applications
Cite this article:
Fitry Tafzi, Surhaini, Dian Wulan Sari, Indra Lasmana Tarigan, Madyawati Latief, Sofia Nurjannah. GC-MS Metabolites of Duku (Lansium domesticum) Leaves and Bark Extract as Anticancer against MCF-7, Hela Cancer Cell, and Molecular Docking Revealed its Potential. Research Journal Pharmacy and Technology. 2025;18(8):3829-0. doi: 10.52711/0974-360X.2025.00551
Cite(Electronic):
Fitry Tafzi, Surhaini, Dian Wulan Sari, Indra Lasmana Tarigan, Madyawati Latief, Sofia Nurjannah. GC-MS Metabolites of Duku (Lansium domesticum) Leaves and Bark Extract as Anticancer against MCF-7, Hela Cancer Cell, and Molecular Docking Revealed its Potential. Research Journal Pharmacy and Technology. 2025;18(8):3829-0. doi: 10.52711/0974-360X.2025.00551 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-8-54
REFERENCES:
1. Yulita KS. Genetic variations of Lansium domesticum Corr. accessions from Java, Sumatra and Ceram based on Random Amplified Polymorphic DNA fingerprints. Biodiversitas J Biol Divers. 2011; 12(3): 125-130. doi:10.13057/biodiv/d120301
2. Lubis MF, Hasibuan PA, Syahputra H, Astyka R. A Review on Phytochemicals and Pharmacological Activities as Ethnomedicinal Uses of Duku (Lansium domesticum Corr.). Open Access Maced J Med Sci. 2022; 10(F): 57-65. doi:10.3889/oamjms.2022.8394
3. Abdallah HM, Mohamed GA, Ibrahim SRM. Lansium domesticum—A Fruit with Multi-Benefits: Traditional Uses, Phytochemicals, Nutritional Value, and Bioactivities. Nutrients. 2022; 14(7): 1-42. doi:10.3390/nu14071531
4. Eryani, Fenti, Muswita, Yelianti U. Effect of Green Fertilizer of Gamal Plant (Gliricidia sepium (Jacq) DC.) On the Growth of Duku (Lansium domesticum Corr.). Int J Ecophysiol. 2020; 2(1): 55-61. doi:10.32734/ijoep.v2i1.3989
5. Khalili RMA, Noratiqah JM, Norhaslinda R, et al. Cytotoxicity effect and morphological study of different duku(Lansium domesticum corr.) Extract towards human colorectal adenocarcinoma cells line (HT-29). Pharmacogn J. 2017; 9(6): 757-761. doi:10.5530/pj.2017.6.119
6. Manosroi A, Chankhampan C, Manosroi W, Manosroi J. Anti-proliferative and matrix metalloproteinase-2 inhibition of Longkong (Lansium domesticum) extracts on human mouth epidermal carcinoma. Pharm Biol. 2013; 51(10): 1311-1320. doi:10.3109/13880209.2013.790064
7. Fadhilah K, Wahyuono S, Astuti P. Fractions and isolated compounds from lansium domesticum fruit peel exhibited cytotoxic activity against t-47d and hepg2 cell lines. Biodiversitas. 2021; 22(9): 3743-3748. doi:10.13057/biodiv/d220918
8. Lukitaningsih E, Saputro AH, Widiasri M, Khairunnisa N, Prabaswari N, Kuswahyuningsih R. In Vitro Antiaging Analysis of Topical Pharmaceutical Preparation Containing Mixture of Strawberry Fruit, Pomelo Peel, and Langsat Fruit Extracts. Indones J Chemom Pharm Anal. 2020; 1(1): 53. doi:10.22146/ijcpa.603
9. Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A. J. Determination of free radical scavenging, antioxidative DNA damage activities and phytochemical components of active fractions from lansium domesticum corr. Fruit. Nutrients. 2015; 7(8): 6852-6873. doi:10.3390/nu7085312
10. Marfori EC, Kajiyama SI, Fukusaki E ichiro. Lansioside D , a new triterpenoid glycoside antibiotic from the fruit peel of Lansium domesticum Correa. 2015; 3(5): 140-143.
11. Manosroi A, Jantrawut P, Sainakham M, Manosroi W, Manosroi J. Anticancer activities of the extract from Longkong (Lansium domesticum) young fruits. Pharm Biol. 2012; 50(11): 1397-1407. doi:10.3109/13880209.2012.682116
12. Omar S, Marcotte M, Fields P, et al. Antifeedant activities of terpenoids isolated from tropical Rutales. J Stored Prod Res. 2007; 43(1): 92-96. doi:10.1016/j.jspr.2005.11.005
13. Aly SH, Elbadry AMM, Doghish AS, El-Nashar HAS. Unveiling the pharmacological potential of plant triterpenoids in breast cancer management: an updated review. Naunyn Schmiedebergs Arch Pharmacol. 2024; 397(8): 5571-5596. doi:10.1007/s00210-024-03054-2
14. Syamsuardi S, Chairul C, Murni P. Analysis of Genetic Impurity of An Original Cultivar Duku (Lansium parasiticum (Osbeck.) K.C. Sahni & Bennet.), from Jambi, Indonesia Using ITS and MatK Gene. Int J Environ Agric Biotechnol. 2018; 3(2): 441-446. doi:10.22161/ijeab/3.2.16
15. Ibrahim A, Siswandono S, Bambang PEW. Anticancer activity of Peronema canescens Jack leaves extracts against human cells: HT-29 and HeLa in vitro. Res J Pharm Technol. 2022; 15(10): 4739-4745. doi:10.52711/0974-360X.2022.00796
16. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-tieulent J, Jemal A. Global Cancer Statistics, 2012. CA a cancer J Clin. 2015; 65(2): 87-108. doi:10.3322/caac.21262.
17. Dhungana BR, Jyothi Y, Kuntal D, Radhika P. Antioxidant and Anticancer potential of Methanolic extract of Kigelia pinnata fruits against DENA induced Hepatocellular Carcinoma in rats . Res J Pharm Technol. 2019; 12(6): 2784. doi:10.5958/0974-360x.2019.00468.2
18. Latief M, Lizawati, Tarigan IL, Muhaimin, Sari PM. Screening of antibiotic candidates from nine medicinal plants Jambi Province. In: AIP Conference Proceedings. Vol 080004.; 2023.
19. Kumar V, Sharma A, Kaur H, Thukral AK, Bhardwaj R. Phytochemical profiling of the leaves of Chenopodium and Polygonum using GC-MS. Res J Pharm Technol. 2015; 8(12): 1629-1632. doi:10.5958/0974-360X.2015.00292.9
20. Aravind R, Bindu AR, Bindu K, Alexeyena V. GC-Ms analysis of the bark essential oil of cinnamomum malabatrum (burman. f) blume. Res J Pharm Technol. 2014; 7(7): 754-759.
21. Salazar-Aranda R, Perez-Lopez LA, Lopez-Arroyo J, Alanis-Garza BA, Waksman De Torres N. Antimicrobial and antioxidant activities of plants from northeast of Mexico. Evidence-based Complement Altern Med. 2011; 2011. doi:10.1093/ecam/nep127
22. Seena H, Kannappan N, Kumar PM. In vitro antioxidant and anticancer activity of methanolic extract of Alangium salvifolium subsp. hexapetalum ( Wangerin). Res J Pharm Technol. 2020;13(8):3715. doi:10.5958/0974-360x.2020.00657.5
23. Semreen MH, Alniss H, Cacciatore S, et al. GC–MS based comparative metabolomic analysis of MCF-7 and MDA-MB-231 cancer cells treated with Tamoxifen and/or Paclitaxel. J Proteomics. 2020; 225(June): 103875. doi:10.1016/j.jprot.2020.103875
24. Singh M, Prathapan A, Nagori K, Ishwarya S, Raghu K. Cytotoxic and Antimicrobial Activity of Methanolic Extract of Boerhaavia diffusa L. Res J Pharm Technol. 2010; 3(4): 1061-1063.
25. Yulianto W, Andarwulan N, Giriwono PE, Pamungkas J. Bioactive Compounds From Torbangun [Plectranthus amboinicus (Lour.) SPRENG] Chloroform Fraction Induce Apoptosis In Breast Cancer (MCF-7 Cells) In VitrO. Trad Med J. 2017; 22(1): 37. doi:10.22146/tradmedj.24312
26. Manna A, Laksitorini MD, Hudiyanti D, Siahaan P. Molecular Docking of Interaction between E-Cadherin Protein and Conformational Structure of Cyclic Peptide ADTC3 (Ac-CADTPC-NH2) Simulated on 20 ns. J Kim Sains dan Apl. 2017; 20(1): 30-36. doi:10.14710/jksa.20.1.30-36
27. Latief M, Sutrisno, Dasrinal E, Safitri W, Tarigan IL. Immunomodulator Activity of 5,7-dihydroxy isoflavones and β-Sitosterol from Peronema canescens Jack Leaves Methanol and Ethyl Acetate Extract. In: Proceedings of the 4th Green Development International Conference (GDIC 2022). Atlantis Press SARL. 2023: 558-572. doi:10.2991/978-2-38476-110-4_57
28. Chauhan R, Singh N, Abraham J. Bioactivity and Molecular Docking of Secondary Metabolites produced by Streptomyces xanthochromogenes JAR5 . Res J Pharm Technol. 2015; 8(3): 300. doi:10.5958/0974-360x.2015.00050.5
29. Hussain G, Rasul A, Anwar H, et al. Role of plant derived alkaloids and their mechanism in neurodegenerative disorders. Int J Biol Sci. 2018; 14(3): 341-357. doi:10.7150/ijbs.23247
30. Weng A, Thakur, Melzig, Fuchs. Chemistry and pharmacology of saponins: special focus on cytotoxic properties. Bot Targets Ther. 2011; (October): 19. doi:10.2147/btat.s17261
31. Manosroi A, Jantrawut P, Sainakham M, et al. Anticancer activities of the extract from Longkong ( Lansium domesticum ) young fruits nticancer activities of the extract from Longkong. 2012; 0209(February). doi:10.3109/13880209.2012.682116
32. Ragasa CY, Labrador P, Rideout JA. Antimicrobial terpenoids from Lansium domesticum Antimicrobial Terpenoids from Lansium domesticum. 2014; 89(May): 101-105.
33. Wong KC, Wong SW, Siew SS, Tie DY. Volatile constituents of the fruits of lansium domesticum correa (Duku and Langsat) and baccaurea motleyana (Muell. Arg.) Muell. Arg. (Rambai). Flavour Fragr J. 1994; 9(6): 319-324. doi:10.1002/ffj.2730090608
34. Šukele R, Lauberte L, Kovalcuka L, et al. Chemical Profiling and Antioxidant Activity of Tanacetum vulgare L. Wild-Growing in Latvia. Plants. 2023; 12(10). doi:10.3390/plants12101968
35. Sami FJ, Soekamto NH, Okino T, Firdaus, Latip J. A Flavonoid compound of Turbinaria decurrens Bory with The Potential Antioxidant and Anticancer Activity. Res J Pharm Technol. 2021; 14(12): 6207-6210. doi:10.52711/0974-360X.2021.01074
36. Yahia M, Yahia M, Benhouda A. Antitumor Activity of Methanolic Fractions Extracted From the Aerial Part of Algerian Hyoscyamus albus and apoptotic cell Aspect Visualization . Res J Pharm Technol. 2017; 10(11): 3676. doi:10.5958/0974-360x.2017.00666.7
37. Seifaddinipour M, Farghadani R, Namvar F, Mohamad J, Kadir HA. Cytotoxic effects and anti-angiogenesis potential of pistachio (Pistacia vera L.) hulls against MCF-7 human breast cancer cells. Molecules. 2018; 23(1). doi:10.3390/molecules23010110
38. Latief M, Muhaimin, Heriyanti, Tarigan IL, Sutrisno. Determination Antioxidant Activity of Coffea Arabica, Coffea Canephora, Coffea Liberica and Sunscreens Cream Formulation for Sun Protection Factor (SPF). Pharmacogn J. 2022; 14(2): 335-342. doi:10.5530/pj.2022.14.43
39. Rahmadani L, Saputri T. Cytotoxic of Ethanol Extract of Leaves and Bark Mareme (Glochidion Arborescens Blume.) Against 4T1 Cancer Cells. Pharmacon. 2024; 21(1): 19-26.
40. Fitri IY, Kurniawanti K, Syahbirin G, Sugita P. Antioxidant Activity, Cytotoxicity, and Identification of Secondary Metabolites of Kigelia africana from Waterpark Platinum Riau. Indo J Chem Res. 2023; 11(2): 142-155. doi:10.30598//ijcr.2023.11-iis
41. Chaudhry GES, Md Akim A, Sung YY, Sifzizul TMT. Cancer and apoptosis: The apoptotic activity of plant and marine natural products and their potential as targeted cancer therapeutics. Front Pharmacol. 2022; 13(August): 1-24. doi:10.3389/fphar.2022.842376
42. Fadilah F, Wiyono L, Edina BC, et al. In Silico Study and In Vitro test of Extract Kaempferia pandurata Roxb. as Anti ER (+) Breast Cancer Cell Line MCF-7 . Res J Pharm Technol. 2019; 12(5): 2391. doi:10.5958/0974-360x.2019.00400.1
43. Setzer WN, Park G, Agius BR, Stokes SL, Walker TM, Haber WA. Chemical compositions and biological activities of leaf essential oils of twelve species of Piper from Monteverde, Costa Rica. Nat Prod Commun. 2008; 3(8): 1367-1374. doi:10.1177/1934578x0800300823
44. Gong JE, Kim JE, Lee SJ, et al. Anti-Cancer Effects of α-Cubebenoate Derived from Schisandra chinensis in CT26 Colon Cancer Cells. Molecules. 2022; 27(3). doi:10.3390/molecules27030737
45. Turkez H, Celik K, Togar B. Effects of copaene, a tricyclic sesquiterpene, on human lymphocytes cell in vitro. Cytotechnology. 2014; 66: 579-603.