Author(s):
Lakireddy Saraswathi, Koduru Swathi, Mitta Chaitanya, Divya Yada, Kalepu Swathi, Meruva Sathish Kumar, Bairam Ravindar, Sandhya Donthula
Email(s):
sandhya.donthula99@gmail.com
DOI:
10.52711/0974-360X.2025.00605 
Address:
Lakireddy Saraswathi1, Koduru Swathi2, Mitta Chaitanya2, Divya Yada3, Kalepu Swathi2, Meruva Sathish Kumar4, Bairam Ravindar5, Sandhya Donthula1*
1Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Dhullapally, Secunderabad, Telangana – 500100.
2Department of Pharmaceutical Chemistry, Bojjam Narasimhlu College of Pharmacy for Women, Vinay Nagar, Saidabad, Hyderabad – 500059, Telangana, India.
3Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Maisammaguda, Dhullapally, Secunderabad, Telangana – 500100.
4Department of Pharmaceutical Analysis, MNR College of Pharmacy, Fasalwadi, Narsapur Road, Sangareddy - 502294, Telangana, India.
5Department of Pharmaceutical Chemistry, Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Siddipet - 502277, Telangana, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 9,
Year - 2025
ABSTRACT:
The aim of the present investigation was to develop, validate and compare a spectrophotometric and a high performance liquid chromatography method for estimating voriconazole in bulk and tablet dosage form. Spectrophotometry and high performance liquid chromatography were carried out using standard instrumental parameters, which were optimized. Both methods were validated in terms of linearity, accuracy, precision, robustness, ruggedness and stability according to the ICH guidelines. The optimized ratio of mobile phase in high performance liquid chromatography under low pressure gradient mode was 60:40% v/v of acetonitrile:orthophosphoric acid 0.1%, which provide a sharp peak with a short retention time of 2.841minutes. In UV spectrophotometric analysis iso-propyl alcohol as a solvent gave adequate molar absorptivity at a ?max of 256nm. Results indicated that both spectrophotometric and high performance liquid chromatography methods were linear, precise, accurate, rugged and robust with RSD values less than 2% and percent recovery was within the standard limits (90-110%). Both the methods were found to be statistically non-significant at 95% confidence intervals (p<0.05) with respect to each other. The proposed methods were found to be highly effective and could be used for quantification of voriconazole in bulk and a tablet formulations for routine analysis.
Cite this article:
Lakireddy Saraswathi, Koduru Swathi, Mitta Chaitanya, Divya Yada, Kalepu Swathi, Meruva Sathish Kumar, Bairam Ravindar, Sandhya Donthula. Comparative UV Spectrophotometric and HPLC Method for Estimation of Voriconazole in Bulk and a Tablet Dosage Form. Research Journal of Pharmacy and Technology. 2025;18(9):4209-4. doi: 10.52711/0974-360X.2025.00605
Cite(Electronic):
Lakireddy Saraswathi, Koduru Swathi, Mitta Chaitanya, Divya Yada, Kalepu Swathi, Meruva Sathish Kumar, Bairam Ravindar, Sandhya Donthula. Comparative UV Spectrophotometric and HPLC Method for Estimation of Voriconazole in Bulk and a Tablet Dosage Form. Research Journal of Pharmacy and Technology. 2025;18(9):4209-4. doi: 10.52711/0974-360X.2025.00605 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-9-25
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