Author(s):
Komalakshi K. V., Divakar M. S., Swathi, Nityasree B. R., Avinash Arasidda Kamble, Raju Krishna Chalannavar
Email(s):
rajuc006@gmail.com , drrajkc@gmail.com
DOI:
10.52711/0974-360X.2025.00611 
Address:
Komalakshi K. V.1, Divakar M. S.2, Swathi1, Nityasree B. R.1, Avinash Arasidda Kamble3, Raju Krishna Chalannavar1*
1Department of Applied Botany, Mangalore University, Mangalagangotri, Karnataka 574199, India.
2Department of Biosciences, Mangalore University, Mangalagangotri, Karnataka 574199, India.
3Department of Industrial Chemistry, Mangalore University, Mangalagangotri, Karnataka 574199, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 9,
Year - 2025
ABSTRACT:
Allophylus concanicus Radlk belongs to the Sapindaceae family and is known for its wide range of therapeutic applications. In this study, we investigated the phytoconstituents, GC-MS profile, and cytotoxic effects of the methanol extract of A. concanicus leaves (MEACL). The leaf methanol crude extract was subjected to Gas Chromatography–Mass Spectrometry (GC-MS) to analyze bioactive molecules. The toxicity of the samples was evaluated in vitro, and the samples were tested for acute oral toxicity for 14 days in female albino Wistar rats with reference to the guidelines of OECD 425. Preliminary phytochemical screening of the methanol extract of A. concanicus showed the existence of some prominent phytochemicals, GC-MS results for the same extract showed the presence of 24 bioactive molecules, including 4-O-Methylmannose with 39.58% area. The sample showed an IC50 of 382.92µg/mL in an in vitro cytotoxicity study against fibroblast cells (NIH/3T3). In the acute oral toxicity study of Wistar albino rats administered a single oral dose, no significant changes in blood parameters were observed after 14 days. No mortality was observed at a sample concentration of 2000mg/kg per body weight (b.w). An acute oral toxicity study found that the estimated LD50value of the methanol crude extract of A. concanicus leaves was >2000mg/kg b.w and the entire studied were conducted for the first time using A. concanicusplant. The promising results of this study will help us explore the future therapeutic applications of A. concanicus.
Cite this article:
Komalakshi K. V., Divakar M. S., Swathi, Nityasree B. R., Avinash Arasidda Kamble, Raju Krishna Chalannavar. GC-MS Analysis and Evaluation of Acute Oral Toxicity Study on Allophylus concanicus Radlk: A Rare Medicinal Plant. Research Journal of Pharmacy and Technology. 2025;18(9):4255-3. doi: 10.52711/0974-360X.2025.00611
Cite(Electronic):
Komalakshi K. V., Divakar M. S., Swathi, Nityasree B. R., Avinash Arasidda Kamble, Raju Krishna Chalannavar. GC-MS Analysis and Evaluation of Acute Oral Toxicity Study on Allophylus concanicus Radlk: A Rare Medicinal Plant. Research Journal of Pharmacy and Technology. 2025;18(9):4255-3. doi: 10.52711/0974-360X.2025.00611 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-9-31
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