Author(s):
Uttam Prasad Panigrahy, Aswini Kumar Parida, Satyabrata Bhanja
Email(s):
uttampanigrahy@gmail.com
DOI:
10.52711/0974-360X.2025.00612 
Address:
Uttam Prasad Panigrahy1, Aswini Kumar Parida2, Satyabrata Bhanja3
1Faculty of Pharmaceutical Science, Assam Down Town University, Sankar Madhab Path, Gandhi Nagar, Panikhaiti, Guwahati, Assam, India.
2Berhampur University, Berhampur - 760007, Odisha, India.
3RITEE College of Pharmacy, NH-6, Chhatauna, Mandir Hasaud, Raipur, Chhattisgarh, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 9,
Year - 2025
ABSTRACT:
The quantification and analytical validation of Atomoxetine was developed with a stationary phase Phenomenex column C18 (4.6mm×250mm, 5µm) and LC1220 Agilent HPLC instrument equipped with variable wavelength detector at 270nm and Acetonitrile: Water containing 0.2% of triethylamine (85:15, v/v) was used as mobile phase passed at a flow rate 1mL/min. Elution takes place at retention time of 2.283min. Validation of method were performed as per ICH guidelines which shows linearity concentration range from 10-125µg/mL amid Correlation coefficient=0.999 with Y = 63164x+64858 regression equation obtained. LOD and LOQ of Atomoxetine were 0.07µg/mL and 0.21µg/mL correspondingly. Recovery of Atomoxetine was obtained 99.83-100.14%.
Cite this article:
Uttam Prasad Panigrahy, Aswini Kumar Parida, Satyabrata Bhanja. Quantification and Analytical Validation of Atomoxetine by Liquid Chromatographic Method through Isocratic Separation in Pharmaceutical Dosage Forms. Research Journal of Pharmacy and Technology. 2025;18(9):4264-8. doi: 10.52711/0974-360X.2025.00612
Cite(Electronic):
Uttam Prasad Panigrahy, Aswini Kumar Parida, Satyabrata Bhanja. Quantification and Analytical Validation of Atomoxetine by Liquid Chromatographic Method through Isocratic Separation in Pharmaceutical Dosage Forms. Research Journal of Pharmacy and Technology. 2025;18(9):4264-8. doi: 10.52711/0974-360X.2025.00612 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-9-32
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