Author(s):
Zaineb Z. Abduljaleel, Khalid K. Al-Kinani
Email(s):
zainab.abd2200@copharm.uobaghdad.edu.iq
DOI:
10.52711/0974-360X.2025.00625 
Address:
Zaineb Z. Abduljaleel1,2*, Khalid K. Al-Kinani2
1Ministry of Health, Karbala Health Directorate, Karbala, Iraq.
2Department of Pharmaceutics, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
*Corresponding Author
Published In:
Volume - 18,
Issue - 9,
Year - 2025
ABSTRACT:
A simple, sensitive, and robust spectrophotometric method was developed and validated for the quantification of acemetacin in phosphate buffer pH 6.8 and ethanol. Linearity was achieved in the range of 5–55µg/mL with regression coefficient 0.9996 for phosphate buffer and 0.9996 for ethanol. The method demonstrated low limits of detection and quantification, which were 0.29µg/mL and 0.89µg/mL for phosphate buffer, and 1.248µg/mL and 3.78µg/mL for ethanol, respectively. Accuracy studies revealed mean recoveries of 97.65%–103.68% in phosphate buffer and 96.52%–101.68% in ethanol, with relative standard deviations below 0.78%, ensuring method reliability. Precision assessments, including intraday and interday studies, confirmed the reproducibility of the method with RSD values <3%, in compliance with FDA and ICH guidelines. The excellent sensitivity, accuracy, and precision of the proposed method make it a valuable tool for the reliable determination of acemetacin in both buffer- and ethanol-based analytical systems.
Cite this article:
Zaineb Z. Abduljaleel, Khalid K. Al-Kinani. Development and Validation of UV Spectrophotometric Method for The Determination of Acemetacin. Research Journal of Pharmacy and Technology. 2025;18(9):4363-8. doi: 10.52711/0974-360X.2025.00625
Cite(Electronic):
Zaineb Z. Abduljaleel, Khalid K. Al-Kinani. Development and Validation of UV Spectrophotometric Method for The Determination of Acemetacin. Research Journal of Pharmacy and Technology. 2025;18(9):4363-8. doi: 10.52711/0974-360X.2025.00625 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-9-45
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