Author(s):
Fatima Zohra Khiat, Hanane Sihem Sebaa, Ahlem Karbab, Nuha Sweidan, Noureddine Charef, Djabeur Abderrezak
Email(s):
fatimazohra.khiat@univ-usto.dz , ahlem.karbab@univ-setif.dz
DOI:
10.52711/0974-360X.2026.00028 
Address:
Fatima Zohra Khiat1, Hanane Sihem Sebaa1, Ahlem Karbab2*, Nuha Sweidan3, Noureddine Charef2*, Djabeur Abderrezak1
1Laboratoire de Productions, Valorisations Végétales et Microbiennes LP2VM, Département de Biotechnologie, Faculté des Sciences de la Nature et de La Vie, Université des Sciences et de la Technologie d'Oran Mohamed Boudiaf, USTO-MB, BP 1505, El M'naouer, Oran, 31000, Algérie.
2Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Science, Setif-1 University, Ferhat Abbas 19000 Algeria.
3Department of Chemistry, Faculty of Arts and Sciences, University of Petra, Amman 961343, Jordan.
*Corresponding Author
Published In:
Volume - 19,
Issue - 1,
Year - 2026
ABSTRACT:
Argania spinosa L. (Argan tree), also known as “iron tree," is a woody species belonging to the Sapotaceae family and is endemic in North-West Africa. In Algeria, it is found as wild in different regions (Tindouf and Stidia), where they are characterized by a great floristic diversity and it is used by the local populations for therapeutic purposes. The fractionation of the Argan pulp fruit allowed to obtain: hydromethanol (HMTE), ethyle acetate (EATE) and butanol extracts (BuTE) from Tindouf and hydromethanol (HMSE), ethyle acetate (EASE) and butanol extracts (BuSE) from Stidia regions, respectively. The objective of this study is to evaluate the total phenolic, flavonoids and condensed tannins contents using spectrophotometric assays. Qualitative analyses were performed. Furthermore, in vitro antioxidant activity using DPPH radical scavenging activity was evaluated. From the qualitative phytochemical screening, the results revealed the presence of alkaloids, polyphenols, free quinone, anthraquinones, terpenoids, tannins and coumarins in the extracts of A. spinosa L. from the two regions. In quantitative analysis, the results revealed that HMSE presented a high amount of total phenolic and condensed tannins contents with values of 172.55 ± 1.46 µg gallic acid equivalent and 31.04 ± 0.75 µg catechin equivalent/mg dry extract, respectively. This extract show an essential effect toward DPPH-scavenging assay with an IC50 of 05.80 ± 0.00 µg/mL, followed by HMTE with an IC50 of 08.40 ± 0.00 µg/mL, respectively. In conclusion, the Argania spinosa L. fruit provides effective natural antioxidants for the patient, as well as alternatives for natural antioxidants.
Cite this article:
Fatima Zohra Khiat, Hanane Sihem Sebaa, Ahlem Karbab, Nuha Sweidan, Noureddine Charef, Djabeur Abderrezak. Comparative Analysis of Phenolic Contents and In vitro Antioxidant Activity of Argania spinosa (L.) Skeels Sub-fractions Pulp from two Algerian Regions. Research Journal of Pharmacy and Technology. 2026;19(1):186-2. doi: 10.52711/0974-360X.2026.00028
Cite(Electronic):
Fatima Zohra Khiat, Hanane Sihem Sebaa, Ahlem Karbab, Nuha Sweidan, Noureddine Charef, Djabeur Abderrezak. Comparative Analysis of Phenolic Contents and In vitro Antioxidant Activity of Argania spinosa (L.) Skeels Sub-fractions Pulp from two Algerian Regions. Research Journal of Pharmacy and Technology. 2026;19(1):186-2. doi: 10.52711/0974-360X.2026.00028 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-1-28
REFERENCES:
1. Ouedraogo WJ. Yerbanga RS. Meda R. Ouedraogo JB. Ouedraogo GA. Revue des plantes médicinales a potentiel aromatique du Burkina Faso: Medicinal plants of aromatic potential from Burkina Faso: a Review. Health Research in Africa. 2024; 2(8). doi.org/10.5281/hra.v2i8.5940
2. Faruk MD. Saha D. Chowdhury S. Paul S. Kabir G. In vitro antioxidant activity of methanolic aerial part extract of Mimosa pudica. Research Journal Pharmacology and Pharmacodynamics. 2012; 4(4): 202-205.
3. Ashfaq MH. Siddique A. Shahid S. Antioxidant activity of Cinnamon zeylanicum: (A Review). Asian Journal of Pharmaceutical Research. 2021; 11(2): 106-6. doi.org/10.52711/2231-5691.2021.00021
4. Ghalem S. Kechairi R. Hassani F. Abdelli I. [Study title not available]. Bangladesh Journal of Botany. 2024; 53(3). doi.org/10.3329/bjb.v53i3.76255
5. Mouafik M. Fouad M. Audet FA. El Aboudi A. Comparative analysis of multi-source data for machine learning-based LAI estimation in Argania spinosa. Advances in Space Research. 2024; 73(10): 4976-4987. doi.org/10.1016/j.asr.2024.02.031
6. Lafdil FZ. Amirou A. Bnouham M. Legssyer A. Ziyyat A. Seddik R. Mekhfi H. Argania spinosa leaves and branches: antiaggregant, anticoagulant, antioxidant activities and bioactive compounds quantification. Advances in Space Research. 2023. [Article in press]. doi.org/10.1016/j.asr.2024.02.031
7. Hilali M. Monfalouti HE. Kartah BE. Evaluation of the chemical composition of Argan (Argania spinosa L.) oil according to its extraction method, origin of production and altitude. Online Journal of Animal and Feed Research. 2020; 10(3): 111-118. doi.org/10.36380/scil.2020.ojafr15
8. Ibourki M. Gharby S. Guillaume D. Laknifli A. El Hammadi A. Charrouf Z. Profiling of mineral elements and heavy metals in argan leaves and fruit by-products using inductively coupled plasma optical emission spectrometry and atomic absorption spectrometry. Chemical Data Collections. 2021; 35: e100772. doi.org/10.1016/j.cdc.2021.100772
9. Guillaume D. Pioch D. Charrouf Z. Argan [Argania spinosa (L.) Skeels] oil. In: Ramadan M, editor. Fruit oils: chemistry and functionality. Cham: Springer; 2019; 317-352. doi.org/10.1007/978-3-030-12473-1_16
10. Fellat-Zarrouk K. Smoughen S. Maurin R. Etude de la pulpe du fruit de l'arganier (Argania spinosa) du Maroc. Matière grasse et latex. Actes Ins. Agro. Vet. Rabat. 1987; 7: 17-22.
11. Benaouf Z. Kechairi R. Djorf O. Kies ML. Fruiting and productivity of the Argan (Argania spinosa) tree and evaluation of the nutritional value of its early and late fruits from Western Algeria. International Journal of Fruit Science. 2023; 23(1): 151-164.
12. Chakhchar A. Ben Salah I. El Kharrassi Y. Filali-Maltouf A. El Modafar C. Lamaoui M. Agro-fruit-forest systems based on argan tree in Morocco: A review of recent results. Frontiers in Plant Science. 2022. 12:783615. doi.org/10.3389/fpls.2021.783615
13. El Idrissi Y. El Moudden H. Harhar H. Zarrouk A. Tabyaoui M. Comparison and correlation of phytochemical content with antioxidant potential of different parts of Argan tree, Argania spinosa L. Caspian Journal of Environmental Sciences. 2021; 19(2): 261-266. doi.org/10.22124/cjes.2021.4743
14. Cherki M. Drissi A. Derouiche A. El Messal M. Bamou Y. Idrissi-Oudghiri A. Khallil A. Adlouni A. Influence of argan oil administration on lipid peroxidation and paraoxonase activities in healthy Moroccan men. Atherosclerosis Supplements. 2003; 4(2): 282.
15. Karbab A. Mokhnache K. Arrar L. Baghiani A. Khennouf S. Charef N. Fractionation, phytochemical screening and free radical scavenging capacity of different subfractions from Pituranthos scoparius roots. Journal of Drug Delivery and Therapeutics. 2020; 10: 133-136. https://jddtonline.info/index.php/jddt/article/view/4100
16. Karbab A. Mokhnache K. Ouhida S. Charef N. Djabi F. Arrar L. Mubarak MS. Anti-inflammatory, analgesic activity, and toxicity of Pituranthos scoparius stem extract: An ethnopharmacological study in rat and mouse models. Journal of Ethnopharmacology. 2020; 258: 112936. doi.org/10.1016/j.jep.2020.112936
17. Karbab A. Charef N. Arrar L. Phenolic contents, in vitro antioxidant, and in vivo anti-inflammatory studies of aqueous extract from Pituranthos scoparius (Coss. and Dur.) growing in Algeria. Iranian Journal of Pharmacology and Therapeutics. 2019; 17: 1-7.
18. Amari S. Karbab A. Charef N. Arrar L. Mubarak MS. Anti-urolithiatic, antibacterial, anti-inflammatory and analgesic effects of Erica arborea flowers and leaves hydromethanolic extracts: An ethnopharmacological study. Saudi Journal of Biological Sciences. 2023; 30(10): 103785. doi.org/10.1016/j.sjbs.2023.103785
19. Amari S. Karbab A. Arrar L. Charef N. Fractionation, phytochemical screening and antioxidant activity of different sub-fractions from leaves and flowers of Erica arborea L. Turkish Journal of Agriculture - Food Science and Technology. 2023; 11: 830-837. https://doi.org/10.24925/turjaf.v11i4.830-837.5698
20. Kadi H. Kevser A. Ozturk A. Koc F. Ceyhan K. Fragmented QRS complexes are associated with increased left ventricular mass in patients with essential hypertension. Annals of Noninvasive Electrocardiology. 2013; 18(6): 547-554. doi.org/10.1111/anec.12070
21. Zhar N. Naamani K. Dihazi A. Jaiti F. El Keroumi A. Comparative analysis of some biochemical parameters of argan pulp morphotypes (Argania spinosa (L) Skeels) during maturity and according to the continentality in Essaouira region (Morocco). Physiology and Molecular Biology of Plants. 2016; 22: 361-370. doi.org/10.1007/s12298-016-0365-z
22. Alilou H. Akssira M. Chemical composition, antibacterial, antioxidant and insecticidal activities of Moroccan Thapsia transtagana essential oil. Saudi Journal of Biological Sciences. 2021; 28(12): 6756-6764. doi.org/10.1016/j.sjbs.2021.07.052
23. Sebaa HS. Cherifi F. Total phenolic content, flavonoids, tannins and antioxidant activity of endocarps fruits of Argania spinosa (L.) Skeels (Sapotaceae). International Journal of Innovation and Applied Studies. 2021; 32(2): 211-219. http://www.ijias.issr-journals.org/
24. El Babili F. Bouajila J. Fouraste I. Valentin A. Mauret S. Moulis C. Chemical study, antimalarial and antioxidant activities, and cytotoxicity to human breast cancer cells (MCF7) of Argania spinosa. Phytomedicine. 2010; 17(2): 157-160. doi.org/10.1016/j.phymed.2009.05.014
25. Karbab A. Charef N. Zarga MHA. Qadri MI. Mubarak MS. Ethnomedicinal documentation and anti-inflammatory effects of n-butanol extract and of four compounds isolated from the stems of Pituranthos scoparius: An in vitro and in vivo investigation. Journal of Ethnopharmacology. 2021; 267: 113488. doi.org/10.1016/j.jep.2020.113488
26. El Idrissi Y. Elouafy Y. El Moudden H. Mghazli N. El Guezzane C. El Yadini A. Tabyaoui M. Evaluation of antioxidant and antimicrobial activity of saponin extracts from different parts of Argania spinosa L. Skeels. Progress in Microbes and Molecular Biology. 2023; 6(1). doi.org/10.36877/pmmb.a0000338
27. Alaoui AA. Azdimousa H. The future of impact investing: strategies for scaling global impact. International Journal of Economic Studies and Management (IJESM). 2024; 4(2): 288-298. doi.org/10.5281/zenodo.10907693
28. Chernane H. Hafidi A. Hadrami IE. Ajana H. Composition phénolique de la pulpe des fruits d’arganier (Argania spinosa L. Skeels) et relation avec leurs caractéristiques morphologiques. Agrochimica. 1999; 43: 137-150.
29. Iftikhar N. Hussain AI. Chatha SAS. Sultana N. Rathore HA. Effects of polyphenol-rich traditional herbal teas on obesity and oxidative stress in rats fed a high-fat–sugar diet. Food Science and Nutrition. 2021; 9(9): 5058-5069. doi.org/10.1002/fsn3.2695
30. Ashfaq MH. Siddique A. Shahid S. Antioxidant activity of Cinnamon zeylanicum: (A Review). Asian Journal of Pharmaceutical Research. 2021; 11(2): 106-116. doi.org/10.52711/2231-5691.2021.00021
31. Afi C. Telmoudi M. Labbassi S. Chabbi N. Hallam J. Msanda F. Ait Aabd N. Assessing the impact of aridity on argan trees in Morocco: Implications for conservation in a changing climate. Resources. 2024; 13(10): 135. doi.org/10.3390/resources13100135
32. El Manfalouti H. Charrouf Z. Belviso S. Ghirardello D. Scursatone B. Guillaume D. Denhez C. Zeppa G. Analysis and antioxidant capacity of the phenolic compounds from argan fruit (Argania spinosa (L.) Skeels). European Journal of Lipid Science and Technology. 2012. 114:. doi.org/10.1002/ejlt.201100209
33. Mechqoq H. El Yaagoubi M. El Hamdaoui A. Momchilova S. Almeida JRGDS. Msanda F. El Aouad N. Ethnobotany, phytochemistry and biological properties of Argan tree (Argania spinosa (L.) Skeels) (Sapotaceae) – A review. Journal of Ethnopharmacology. 2021. 278: 114528. doi.org/10.1016/j.jep.2021.114528
34. Serrafi A. Chegdani F. Bennis F. Kepinska M. The importance of argan oil in medicine and cosmetology. Nutrients. 2024. 16: 3573. doi.org/10.3390/nu16203573