ABSTRACT:
Currently, the potential of 1,2,4-triazole derivatives in the development of anticonvulsants is attracting special attention of researchers, as such compounds can modulate the activity of receptors responsible for the development of seizure syndrome. 1,2,4-triazole derivatives can affect the GABAergic system and ion channels, which play a key role in the mechanisms of seizure development. It is known that 2-[5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-ylthio]-1-(4-chlorophenylethanone) has anticonvulsant activity. Therefore, it is extremely important to develop sensitive and specific methods of analysis on the way to introducing this substance. Development and validation of a simple, economical and eco-friendly UV-spectrophotometric method for the quantitative determination of 2-[5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-ylthio]-1-(4-chlorophenylethanone). A working standard sample of 2-[5-(furan-2-yl)-4-phenyl-4n-1,2,4-triazol-3-ylthio]-1-(4-chlorophenylethanone), ethyl alcohol of grade chemically pure" and the following analytical equipment were used in the study: Specord 250 Plus spectrophotometer, ABT-120-5DM electronic balance, Sonorex Digitec DT100H ultrasonic bath, class A measuring glassware. The optimal parameters for the quantitative determination of 2-[5-(furan-2-yl)-4-phenyl-4n-1,2,4-triazol-3-ylthio]-1-(4-chlorophenylethanone were investigated - the solvent was chosen, the stability of the analyzed solutions in time was studied, and the concentration limits at which the Bouguer-Lambert-Beer law is observed were determined. It was found that the maximum value of light absorption of the substance under study is observed at a wavelength of 280 nm in the medium of ethyl alcohol. On the basis of the obtained data, a spectrophotometric method was developed for the quantitative determination of 2-[5-(furan-2-yl)-4-phenyl-4n-1,2,4-triazol-3-ylthio]-1-(4-chlorophenylethanone by the standard method. The linear dependence within the range of application of the proposed method (80-120%) was estimated. The calculated metrological characteristics confirm the linearity of the method in the concentration range of the substance under study from 0.64 to 0.96 mg/100 ml. The possibility of using the spectrophotometric method in other laboratories with obtaining reliable results by predicting the total uncertainty of the method was proved. As a result of the study, a simple and reliable spectrophotometric method for the quantitative determination of 2-[5-(furan-2-yl)-4-phenyl-4n-1,2,4-triazol-3-ylthio]-1-(4-chlorophenylethanone) by intrinsic absorption was developed. The results were statistically processed, which indicates a high reproducibility of the developed method. The practicality of the proposed method and minimal environmental impact were confirmed using modern analytical tools.