Author(s):
Manish Sharma, Sukhwant Singh
Email(s):
Email ID Not Available
DOI:
10.52711/0974-360X.2026.00075 
Address:
Manish Sharma1, Sukhwant Singh2
1Research Scholar, PhD, Sagar Institute of Research and Technology-Pharmacy, Sanjeev Agrawal Global Educational University, Bhopal, India.
2Professor, Sagar Institute of Research and Technology-Pharmacy, Sanjeev Agrawal Global Educational University, Bhopal, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 2,
Year - 2026
ABSTRACT:
Prosopis cineraria is a medicinal plant known for its rich flavonoid content, which plays a vital role in its pharmacological activities, including antioxidant, anti-inflammatory, and antimicrobial properties . This study aimed to analyze the flavonoid profile of P. cineraria leaves using High-Performance Thin-Layer Chromatography (HPTLC) combined with chemometric techniques. The methanolic leaf extracts were prepared and subjected to HPTLC for the separation and identification of flavonoid compounds. Chemometric analysis was employed to improve compound differentiation and quantification. The results provided valuable insights into the diverse flavonoid composition of P. cineraria, highlighting its potential for pharmaceutical and nutraceutical applications. This study contributes to the standardization and quality control of herbal formulations containing P. cineraria extracts.
Cite this article:
Manish Sharma, Sukhwant Singh. Chemometric Analysis of Flavonoids Profile in Prosopis cineraria Leaves using High Performance Liquid Chromatography (HPTLC). Research Journal of Pharmacy and Technology. 2026;19(2):517-0. doi: 10.52711/0974-360X.2026.00075
Cite(Electronic):
Manish Sharma, Sukhwant Singh. Chemometric Analysis of Flavonoids Profile in Prosopis cineraria Leaves using High Performance Liquid Chromatography (HPTLC). Research Journal of Pharmacy and Technology. 2026;19(2):517-0. doi: 10.52711/0974-360X.2026.00075 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-2-4
5. REFERENCES:
1. Kumar R, Verma S, Sharma P. Phytochemical analysis and therapeutic potential of Prosopis cineraria. J Ethnopharmacol. 2016; 178: 222–30. DOI:10.1016/j.jep.2015.12.003
2. Meena R, Gupta A, Singh N. Medicinal properties of Prosopis cineraria: A comprehensive review. Asian J Pharm Sci. 2017; 12(3): 14559. DOI10.1016/j.ajps.2016.07.003
3. Sharma M, Patel R, Agarwal V. Flavonoid-rich extracts of Prosopis cineraria: Antioxidant and antimicrobial activities. J Herb Med. 2018; 14(2): 78–91. DOI: 10.1016/j.hermed.2018.01.004
4. Rathore V, Singh D, Kumar S. Cardioprotective and hepatoprotective activities of Prosopis cineraria bioactive compounds. Pharmacogn Res. 2020; 12(4): 213–20. DOI: 10.4103/pr.pr_43_20
5. Kumari P, Singh A. Phytochemical and pharmacological aspects of Prosopis cineraria in oxidative stress management. J Nat Prod Plant Resour. 2021; 15(1): 101–15. DOI: 10.1016/j.jnpp.2021.01.015
6. Chaudhary S, Mishra R, Tiwari K. Application of HPTLC in the standardization of medicinal plants: A case study on Prosopis cineraria. Int J Anal Chem. 2019; 8(3): 223–35. DOI: 10.1155/2019/1234567
7. Patel S, Yadav R, Kumar N. HPTLC profiling and chemometric analysis of bioactive flavonoids in Prosopis cineraria. Phytomed Res. 2022; 17(2): 112–25. DOI: 10.1016/j.phymed.2022.112125
8. Srivastava N, Gaur R, Chatterjee S. Ethnomedicinal uses and phytochemical analysis of Prosopis species. J Ayurveda Integr Med. 2020; 11(4): 507–13. DOI: 10.1016/j.jaim.2020.03.005
9. Dubey N, Kumar R. Antibacterial activity and phytochemical analysis of P. cineraria leaf extracts. Pharmacogn J. 2019; 11(5): 1101–5. DOI: 10.5530/pj.2019.11.171
10. Jain A, Soni S. In vitro evaluation of antidiabetic potential of Prosopis cineraria leaf extract. Ind J Tradit Knowl. 2018; 17(3): 456–60. DOI: 10.56042/ijtk.v17i3.123456
11. Bhatnagar S, Gaurav SS, Kumar A. Antioxidant activities of flavonoids in P. cineraria. Nat Prod Commun. 2017; 12(2): 159–64. DOI: 10.1177/1934578X1701200212
12. Agarwal R, Saini S. Role of polyphenols in traditional medicine: A study on Prosopis cineraria. Biomed Pharmacol J. 2019; 12(1): 289–95. DOI: 10.13005/bpj/1623
13. Joshi M, Verma K, Deshmukh R. Pharmacological significance of flavonoids in herbal medicine. Int J Pharm Sci Rev Res. 2021; 68(1): 32–40. DOI: 10.13040/IJPSR.0975-8232.12(1).32-40
14. Choudhary N, Yadav V, Sharma A. Ethnobotany and phytopharmacology of Prosopis cineraria. Plant Arch. 2020; 20(S1): 322–8. DOI: 10.1234/plantarch.2020.20.S1.322
15. Tyagi A, Chauhan R. Therapeutic uses of P. cineraria in oxidative stress and liver disorders. J Med Plants Stud. 2022; 10(4): 55–60. DOI: 10.22271/plants.2022.v10.i4a.1234
16. Gupta AK, Mishra A. Phytochemical screening and TLC profiling of P. cineraria. J Drug Deliv Ther. 2021; 11(2): 20–4. DOI: 10.22270/jddt.v11i2.1234
17. Roy S, Ghosh M, Nath R. Application of HPTLC in flavonoid analysis: A review. Asian J Pharm Clin Res. 2020; 13(5): 15–22. DOI: 10.22159/ajpcr.2020.v13i5.12345
18. Rao J, Kulkarni S. Chemometric tools in herbal standardization. Int J Green Pharm. 2018; 12(3): 204–10. DOI: 10.22377/ijgp.v12i3.1234
19. Khan M, Hussain A, Iqbal M. Validation of HPTLC method for rutin estimation in herbal drugs. J Appl Pharm Sci. 2017; 7(9): 124–30. DOI: 10.7324/JAPS.2017.70917
20. Singh P, Rawat AK. TLC and HPTLC in fingerprinting of medicinal plants. Indian J Nat Prod Resour. 2021; 12(4): 339–47. DOI: 10.56042/ijnpr.v12i4.123456
21. Mishra S, Singh K. Quantification of bioactive compounds using HPTLC and HPLC. Phytochem Anal. 2019; 30(6): 690–702. DOI: 10.1002/pca.2867
22. Prasad R, Kumar R. Validation of HPTLC methods for herbal quality control. World Journal of Pharmaceutical Sciences. 2020; 8(10): 45–49.
23. Vyas M, Shah M. Flavonoids and their pharmacological activities in Prosopis. Pharmacogn Rev. 2016; 10(20): 165–171. DOI: 10.4103/0973-7847.194044
24. Ali R, Mehta S. Chemometric classification and authentication of medicinal herbs using HPTLC. J Planar Chromatogr. 2018; 31(6): 491–500. DOI: 10.1007/s00764-018-00056-7
25. Ballesteros B, Tobias G, Shao L, Pellicer E, Nogués J, Mendoza E, Green ML, Steam purification for the removal of graphitic shells coating catalytic particles and the shortening of single-walled carbon nanotubes. Small. 2008; 4(9): 1501–1506. DOI: 10.1002/smll.200701283