ABSTRACT:
Bioassay guided fractionation technique is step by step separation of phytochemical component based on their physic-chemical properties, and investigating the pharmacological activities, followed by separation and assaying. Cassia tora Linn. is the wild medicinal plant and grows in most parts of India as weeds. In traditional literature, it is found that seeds of Cassia tora Linn. were used for the treatment of diabetes in different parts of the world. It was considered worthwhile to explore the plant for its claimed activity and isolation of phytoconstituents from this plant having antidiabetic potential. Objective is to analyse phytochemical component and antidiabetic potential of seeds of Cassia tora using column chromatography. Methanolic extract processed through bioactive fractionation as water soluble fraction and ethyl acetate fraction. The water soluble fraction was processed through solvent fractionation followed by column chromatography from which five isolates was found that were analyzed for phytochemical and pharmacological screening. The isolate with more potent antidiabetic activity as compared to other isolates was processed through chemical profiling and characterization. The result of antidiabetic potential suggested that methanolic extract possess antidiabetic activity. The phytochemical screening of all five fractions showed the presence of flavanoid, anthraquinone, triterpinoid, sterol and carbohydrate. The flavanoid was present in fraction f-b and fraction f-e. Anthraquinone, triterpenoid and carbohydrate was present in fraction (f-a). On the basis of all phytochemical and pharmacological screening it can be concluded that the antidiabetic potential of seeds of Cassia tora Linn. may be due to the presence of epicatechin using to various modern analytical techniques.
Cite this article:
Shuchi Dave. Bioassay Guided Fractionation and Identification of Antidiabetic Potential of Cassia tora Seeds. Research Journal Pharmacy and Technology. 2026;19(3):1070-4. doi: 10.52711/0974-360X.2026.00151
Cite(Electronic):
Shuchi Dave. Bioassay Guided Fractionation and Identification of Antidiabetic Potential of Cassia tora Seeds. Research Journal Pharmacy and Technology. 2026;19(3):1070-4. doi: 10.52711/0974-360X.2026.00151 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-3-12
6. REFERENCES:
1. American Diabetes Association. Classification and Diagnosis of Diabetes. Diabetes Care. 2016; 39 Suppl 1: S13–S22. https://doi.org/10.2337/dc16-S005
2. Tran N. Pham B. Le L. Bioactive Compounds in Anti-Diabetic Plants: From Herbal Medicine to Modern Drug Discovery. Biology (Basel). 2020 Aug 28; 9(9): 252. doi: 10.3390/biology9090252.
3. Diabetes fact and figures, International diabetes federation Diabetes atlas, Brussels, Belgium. 2019; 19; 226-227
4. Malviya N. Malviya S. Bioassay guided fractionation-an emerging technique influence the isolation, identification and characterization of lead phytomolecules. International Journal of Hospital Pharmacy. 2017; 10.28933/ijhp-2017-07-0901.
5. Ansari A. Meeta B. Evaluation of Phytochemicals of Cassia tora Linn. and it's Cytotoxicity Assay using Brine Shrimp. International Journal of Pharmacognosy and Phytochemical Research. 2017; 9(4); 587-595. doi:10.25258/phyto.v9i2.8132
6. Choudhary M. Gulia Y et al Cassia tora: its chemistry, medicinal uses. Pharmacologyonline. 2011; 3: 78-96.
7. Kumar R. Narasingappa, R. Joshi C. Girish T. Prasada Rao U. Danagoudar, A. Evaluation of Cassia tora Linn. against Oxidative Stress-induced DNA and Cell Membrane Damage. Journal of Pharmacy & Bioallied Sciences. 2017; 9(1): 33–43.
8. Lee Ga. Young et al. Naphthopyrone glucosides from the seeds of Cassia tora with inhibitory activity on advanced glycation end products (AGEs) formation. Archives of Pharmacal Research. 2006; 29(7): 587-90. doi:10.1007/BF02969270
9. Patil U.K. Saraf S. Dixit V.K. Hypolipidemic activity of seeds of Cassia tora Linn. J Ethnopharmacol. 2004; 90(2-3): 249-252. doi:10.1016/j.jep.2003.10.007
10. Kumar Sarwa K, Rudrapal M, Debnath M, Kumar A, Kumar Verma V. Phytochemical and Biological Potential of Cassia tora Linn. Euro. J. Med. Plants. [Internet]. 2014 May 31 [cited 2025 Jul. 28]; 4(8): 946-63. Available from: https://journalejmp.com/index.php/EJMP/article/view/535
11. Wu CH Yen GC. Antigenotoxic properties of Cassia tea (Cassia tora L.): mechanism of action and the influence of roasting process. Life Sci. 2004; 76(1): 85-101. doi:10.1016/j.lfs.2004.07.011
12. Kokate C K. Purohit A P. Gokhale S.B. Pharmacognosy, Nirali Prakashan. 2017; 3: 7.44-7.46.
13. Mahmound R K. Medicinal plants and human needs. Journal of Herbed Pharmacology. 2012; 1(1): 1-2.
14. Nurdiana S. Elizabeth, Jega A.J and Nurul S, Antidiabetic Activity of Azadirachta Excelsa Extract on Alloxan Induced Diabetic Rats. The Open Conference Proccedings Journal. 2013; 4: 269.
15. Somani R. Kasture S. Singhai AK. Antidiabetic potential of Butea monosperma in rats, Fitoterapia. 2006; 77(2): 86-90.
16. Patwardhan B. Vaidhya A. Chorghade M, Ayurveda and Natural Products Drug Discovery. Current Science. 2004; 86: 789-799.
17. Punasiya R. Joshi A. Patidar K. Antidiabetic Effect of an Aqueous Extract of Pomegranate (Punica granatum L.) Peels in Normal and Alloxan Diabetic Rats, Research J. Pharm. and Tech. 2010; 3(1); 272-274.
18. Chauhan H.V. In-Vivo Antidiabetic. Lipid Lowering and Antioxidant Activities of Methanolic Extaract of Lawsonia inermis Leaves. Research J. Pharm. and Tech. 2011; 4(5): 764-767.
19. Iswarya O. Jangam D. Kamireddy S. Gummadi A. Marupilla A. Vaddeswarapu R. Evaluation of Antihyperlipidemic activity of leaves of Cassia tora. Research Journal of Pharmacy and Technology. 2022; 15(2): 741-4.
20. Nachiket S D. Shashikant R P. Sunil A. Nirmal V. et al.A Review on Phytochemical and Pharmacological Profile of Cassia tora Linn. Research J. Pharmacognosy and Phytochemistry. 2009; 1(3): 173-176.
21. Shom P. K., Sunil R. Tripathi K. et al Coupling Antioxidant and Antidiabetic assets of 2, 4-Thiazolidinedione Derivatives. Asian J. Pharm. Ana. 2011; 1(4): 71-73.
22. Sawant S., Randive V. Kulkarni S. R. Lectins from seeds of Abrus precatorius: Evaluation of Antidiabetic and Antihyperlipidemic Potential in Diabetic Rats. Asian J. Pharm. Res. 2017; 7(2): 71-80.
23. Shukla K. Bioassay: An uncomplicated methodologies for ensure safety of Traditional Formulations. Research J. Pharmacognosy and Phytochemistry 2009; 1(1): 01-04.
24. Pradhan D. Madhab Kar D. Ghosh G. Bioassay-guided Fractionation of Anti-microbial principle from Ethanolic Extract of Tinospora cordifolia and its Characterization. Research J. Pharm. and Tech. 2020; 13(3): 1415-1422.
25. K. Radhika, B. Kumaravel, V. Thamizhiniyan, S. Subramanian. Biochemical evaluation of antidiabetic activity of Piper betel leaves extract in alloxan-induced diabetic rats. Asian J. Research Chem. 2013; 6(1): 76-82.
26. Khare S. Tailang M. Sharma A. Shukla T. Hypolipidemic Activity of Leaves and Fruits of Ficus bengalensis in Alloxan Induced Diabetic Rats. Research J. Pharmacognosy and Phytochemistry 2010; 2(4): 293-296.
27. Thennarasan S. Murugesan S. Sivamurugan V. Antidiabetic efficacy of Methanol extract Brown Alga Lobophora variegata on Alloxan stimulated Hyperglycemic Wistar Albino Rats. Asian J. Pharm. Tech. 2017; 7 (3): 157-165.
28. Monago C. C., Nwodo O. Fred C. Antidiabetic Effects of Crude Flavanoid and Alkaloid of Abrus precatorius Linn Seed in Alloxan Diabetic Rabbits. Research J. Pharmacognosy and Phytochemistry. 2010; 2(4): 331-335.