Author(s):
Soumaya El Baraka, Aya Bourdaime, Nihal Bhirich, Ghita Meknassi Salime, Jean-Marie Ouedraogo, Oualid Ziraoui, Brahim Mojemmi, Younes Rahali
Email(s):
s.elbaraka@uca.ac.ma , soumayaelbaraka23@gmail.com
DOI:
10.52711/0974-360X.2026.00176 
Address:
Soumaya El Baraka1,2,3, Aya Bourdaime4,5,6, Nihal Bhirich5,6, Ghita Meknassi Salime4,5,6, Jean-Marie Ouedraogo7, Oualid Ziraoui1,2,3, Brahim Mojemmi5,6, Younes Rahali4,5,6
1Department of Drug Science, Faculty of Medicine and Pharmacy, Cadi Ayyad University, 40000 Marrakesh, Morocco.
2Laboratoire d'excellence d'Innovations en Biotechnologie, Bio-ingénierie Pharmaceutique et Intelligence Artificielle, Faculty of Medicine and Pharmacy, Cadi Ayyad University, 40000 Marrakesh, Morocco.
3Military Ibn Sina University Hospital Center of Marrakesh, 40000 Marrakesh, Morocco.
4Specialties Hospital of Rabat, Ibn Sina University Hospital, 10170 Rabat, Morocco.
5Faculty of Medicine and Pharmacy, Mohammed V University - Rabat, 10170 Rabat, Morocco.
6Ibn Sina Hospital Center of Rabat, Rabat, 10070, Rabat, Morocco.
7Laboratory of Pharmaceutical Sciences, Euromed University of Fez, Eco-Campus UEMF 30000 Fez, Morocco.
*Corresponding Author
Published In:
Volume - 19,
Issue - 3,
Year - 2026
ABSTRACT:
Objective: Hydrogen peroxide is widely used in medical and pharmaceutical applications, but its stability and degradation after expiration are major concerns. The objective of this study is to investigate the stability of hydrogen peroxide in expired capsules and assess its degradation over time, in order to determine its efficacy beyond the expiration date. Methods: A series of experiments were conducted to measure the concentration of hydrogen peroxide in capsules at different time intervals post-expiration (0, 3, 6, and 12 months). The concentration was determined using a permanganometric titration method, and the degradation patterns were analyzed using one-way ANOVA, repeated measures ANOVA, Tukey HSD test, and linear regression analysis to assess the effect of time on hydrogen peroxide concentration. Results: The stability of hydrogen peroxide in the capsules was observed to be high for the first 6 months post-expiration. The mean concentration decreased slightly over time, with the largest decline observed at 12 months. Statistical analysis showed no significant difference between the capsules for up to 6 months, indicating minimal degradation during this period. The linear regression model confirmed a gradual decrease in concentration over time, with a reduction of 0.0193 units per month. Conclusion: Hydrogen peroxide capsules remain effective for at least 6 months after expiration when stored under optimal conditions. The findings suggest that the degradation of hydrogen peroxide is slow and minimal during this period, supporting the potential for using expired hydrogen peroxide capsules within this timeframe, especially in resource-limited settings.
Cite this article:
Soumaya El Baraka, Aya Bourdaime, Nihal Bhirich, Ghita Meknassi Salime, Jean-Marie Ouedraogo, Oualid Ziraoui, Brahim Mojemmi, Younes Rahali. Assessing the Usability of Expired Hydrogen Peroxide for Cold Sterilization: A Simple Analytical Titrimetric Method and Data Analysis for Ensuring Safety and Efficiency in Medical Device Sterilization. Research Journal Pharmacy and Technology. 2026;19(3):1235-9. doi: 10.52711/0974-360X.2026.00176
Cite(Electronic):
Soumaya El Baraka, Aya Bourdaime, Nihal Bhirich, Ghita Meknassi Salime, Jean-Marie Ouedraogo, Oualid Ziraoui, Brahim Mojemmi, Younes Rahali. Assessing the Usability of Expired Hydrogen Peroxide for Cold Sterilization: A Simple Analytical Titrimetric Method and Data Analysis for Ensuring Safety and Efficiency in Medical Device Sterilization. Research Journal Pharmacy and Technology. 2026;19(3):1235-9. doi: 10.52711/0974-360X.2026.00176 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-3-37
REFERENCES:
1. N MR, et al. Abc analysis of hospital pharmacy in a tertiary care teaching hospital. Res J Pharm Technol. 2011; 4(5): 779–81
2. Shah NV, et al. Advances in hospital pharmacy. Res J Pharm Technol. 2017; 10(2):618–24
3. Ganga NP. Polypharmacy: an overview. J Pharm Care. 2022: 229–33
4. Sowjanyaa J, Chandana CS. Clinical practice guidelines on sterilization of endodontic instruments. Res J Pharm Technol. 2018; 11(3): 1039–40
5. Mendhekar KP, et al. A critical review of Dhoopan karma in Ayurveda and Dhoopana of an Ayurvedic herbomineral formulation Yavasarshapadi churna in context of sterilization. Res J Pharm Technol. 2021; 14(11): 6095–100
6. Jitesh S. Knowledge and awareness of sterilization among general dentist. Res J Pharm Technol. 2016; 9(6): 719–21
7. Gamessa TW, et al. Medical devices maintenance campaign: an experience of COVID-19 emergency response in Ethiopia. Res J Pharm Technol. 2021; 14(10): 5475–8
8. Jarow JP, Baxley JH. Medical devices: US medical device regulation. In: Urologic oncology: seminars and original investigations. Elsevier; 2015. p. 128–32
9. Noda M, et al. Evaluation of low-temperature sterilization using hydrogen peroxide gas containing peracetic acid. Biocontrol Sci. 2020; 25(4): 185–91
10. Bhat BB, et al. Medical device industry in India: past to present. Res J Pharm Technol. 2019; 12(12): 5959–62
11. Sravani K, et al. Analytical reagents used in chemical and spectrophotometric analysis. Res J Pharm Technol. 2015; 8(2): 110–7
12. Maris P. Les méthodes de désinfection des surfaces par voie aérienne (DSVA) au peroxyde d’hydrogène sont-elles des alternatives au formaldéhyde? EuroReference – Les Cahiers de la Référence. 2011; (6): 19–23
13. El Baraka S, et al. Evaluating cost savings in cytotoxic leftover management: a prospective study of vial sharing and dose rounding techniques in the Moroccan National Institute of Oncology. J Oncol Pharm Pract. 2024; 30(7): 1152–9
14. El Baraka S, et al. Innovation in hospital pharmacy: modeling the installation of automated dispensing systems based on an oncology hospital experience transfer. J Oncol Pharm Pract. 2025; 31(3): 388–96
15. Schumb WC. Stability of concentrated hydrogen peroxide solutions. Ind Eng Chem. 1949;41(5):992–1003
16. Pandian P, Kalpanapriya D. Two-factor ANOVA technique for fuzzy data having membership grades.ResJPharmTech.2016;9(12):2394–402
17. Bhavyasri D, et al. Marketing approval for medical devices in European Union. Res J Pharm Technol. 2019; 12(1): 391–5
18. Torres CR, et al. Influence of pH on the effectiveness of hydrogen peroxide whitening. Oper Dent. 2014; 39(6): E261–8
19. Trautmann E, et al. Hydrogen peroxide versus sodium hypochlorite: all a matter of pH? J Endod. 2021; 47(2): 297–302
20. Kushwaha P. Significance of stability studies on degradation product. Res J Pharm Technol. 2009; 2(4): 621–7.