Author(s):
M.V.Kumudhavalli, R. Venkatraj, Arun.T, Sadikbasha.A, L.janarthanan, Kumar Mohan
Email(s):
kumudhu27@gmail.com
DOI:
10.52711/0974-360X.2026.00194 
Address:
M.V.Kumudhavalli1*, R. Venkatraj2, Arun.T3, Sadikbasha.A4, L.janarthanan, Kumar Mohan5
Department of Pharmacognosy, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (DU), Salem, Tamilnadu, India-08.
*Corresponding Author
Published In:
Volume - 19,
Issue - 3,
Year - 2026
ABSTRACT:
In this present study the bioanalytical method development and validation of an LC-MS/MS method in human plasma for the quantification of mefenamic acid is described .The method carried out by using liquid-liquid extraction as diclofenac is utilised as an internal standard (IS). Chromatographic separation was carried out on a Hypersil Gold C-18 (100 mm x 4.8 mm, 5 µm) column, using a mobile phase composed of methanol and ammonium acetate buffer in a 70:30 ratio. The flow rate was set at 1 mL/min, and the total runtime was 3 minutes. The analyte was subjected to LC-MS/MS using positive ionization source. The standard curve was linear from 81.94 ng/ml to 10246.69 ng/ml. This assay allows quantification of mefenamic acid at lower concentrations in human plasma. The observed mean recovery value was found to be 68.03% for Mefenamic acid and 94.74% for Diclofenac respectively. From this observed results it was concluded that the developed method was more economic, precise, accurate and lesser time utility for the routine analysis.
Cite this article:
M.V.Kumudhavalli, R. Venkatraj, Arun.T, Sadikbasha.A, L.janarthanan, Kumar Mohan. Bioanalytical method development and validation of mefenamic acid in human plasma by liquid chromatography coupled with tandem mass spectrometry. Research Journal Pharmacy and Technology. 2026;19(3):1351-6. doi: 10.52711/0974-360X.2026.00194
Cite(Electronic):
M.V.Kumudhavalli, R. Venkatraj, Arun.T, Sadikbasha.A, L.janarthanan, Kumar Mohan. Bioanalytical method development and validation of mefenamic acid in human plasma by liquid chromatography coupled with tandem mass spectrometry. Research Journal Pharmacy and Technology. 2026;19(3):1351-6. doi: 10.52711/0974-360X.2026.00194 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-3-55
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