Author(s):
Usha Kiran Reddy Thimmapuram, Nischith Sirawase Shankar, Maged Alkanad, Annegowda Haradur Venkatappa
Email(s):
drannegowda@accp.co.in , magedalkanad@accp.co.in
DOI:
10.52711/0974-360X.2026.00223 
Address:
Usha Kiran Reddy Thimmapuram1,2, Nischith Sirawase Shankar1, Maged Alkanad1*, Annegowda Haradur Venkatappa1*
1Department of Pharmacognosy, Sri. Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B.G. Nagara, Mandya, Karnataka 571448, India.
2Faculty, Department of Pharmacognosy, S V University College of Pharmaceutical Sciences, S V University, Tirupati - 517502, Andhra Pradesh, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 4,
Year - 2026
ABSTRACT:
This study optimized probe sonication-assisted extraction of polyphenols from Terminalia bellerica, Kigelia pinnata, and Aerva lanata using Box-Behnken Design (BBD) and Response Surface Methodology (RSM). The effects of solvent composition (0-100% ethanol), sonication time (5-60min), and amplitude (10-98%) on total phenolic content (TPC) were evaluated. RSM analysis revealed optimal conditions: T. bellerica achieved maximum TPC (602.42±9.23µg GAE/mL) at 62.08% ethanol, 34.39min, and 62.37% amplitude; K. pinnata yielded 60.73±2.56µg GAE/mL at 44.26% ethanol, 27.03min, and 55.41% amplitude; and A. lanata showed highest TPC (34.24±1.28µg GAE/mL) at 46.54% ethanol, 20min, and 44.79% amplitude. The validated model confirmed probe sonication as an efficient, scalable method for polyphenol extraction, highlighting its potential for pharmaceutical and industrial applications.
Cite this article:
Usha Kiran Reddy Thimmapuram, Nischith Sirawase Shankar, Maged Alkanad, Annegowda Haradur Venkatappa. Optimization of Polyphenol Extraction from Three Medicinal Plants using Probe Sonication: A Box-Behnken Design and Response Surface Methodology Approach. Research Journal of Pharmacy and Technology. 2026;19(4):1557-6. doi: 10.52711/0974-360X.2026.00223
Cite(Electronic):
Usha Kiran Reddy Thimmapuram, Nischith Sirawase Shankar, Maged Alkanad, Annegowda Haradur Venkatappa. Optimization of Polyphenol Extraction from Three Medicinal Plants using Probe Sonication: A Box-Behnken Design and Response Surface Methodology Approach. Research Journal of Pharmacy and Technology. 2026;19(4):1557-6. doi: 10.52711/0974-360X.2026.00223 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-4-12
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