Author(s):
Shakeel Memon, Neha Mohite, Kanchan Chauhan, Tabssum Hangad, Dilnawaz Pathan
Email(s):
memon.shakeel@gmail.com
DOI:
10.52711/0974-360X.2026.00231 
Address:
Shakeel Memon1, Neha Mohite4*, Kanchan Chauhan1, Tabssum Hangad2, Dilnawaz Pathan3
1Associate Professor, M. C. E. Society’s Allana College of Pharmacy, Azam Campus, Pune, Maharashtra, India.
2Assistant Professor, M. C. E. Society’s Allana College of Pharmacy, Azam Campus, Pune, Maharashtra, India.
3HOD, Department of Pharmaceutics, Trinity College of Pharmacy, Pune, Maharashtra, India.
4Assistant Professor, Adarsh College of Pharmacy, Vita, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 4,
Year - 2026
ABSTRACT:
The analytical investigation work illustrates the expansion along with validation of a HPTLC chromatographic technology intended for instantaneous evaluation drugs, Dapagliflozin (DAPA) along with Vildagliptin (VILDA) within pharmaceutical formulation. Utilizing aluminum-backed TLC plates (10 × 10 ) already coated with 250µm layer of silica gel having pore size 60 with fluorescent indicator F254 while in mobile phase and a mobile phase made up of n-Hexane: EthylAcetate: Methanol (3:6:1 v/v/v), the chromatography was execute. By densitometrically scanning plates at 225nm and 243nm with Camag TLC scanner, the drug’s quantitative determination was carried out. The linearity with accuracy along with precision with that detection limit and quantitation limit of as well as robustness of established technique approach was every confirmed. By employing an ultraviolet detector with a wavelength of 225 and 243 nm, DAPA and VILDA were quantified using this method respectively. With supplementary peaks at Rf values of 0.44±0.12 and 0.72±0.23 for DAPA and VILDA correspondingly, both drugs demonstrated deterioration.
Cite this article:
Shakeel Memon, Neha Mohite, Kanchan Chauhan, Tabssum Hangad, Dilnawaz Pathan. Development and Validation of a HPTLC Method to Estimate Dapagliflozin and Vildagliptinalong with Bulk and Marketed Formulation. Research Journal of Pharmacy and Technology. 2026;19(4):1618-4. doi: 10.52711/0974-360X.2026.00231
Cite(Electronic):
Shakeel Memon, Neha Mohite, Kanchan Chauhan, Tabssum Hangad, Dilnawaz Pathan. Development and Validation of a HPTLC Method to Estimate Dapagliflozin and Vildagliptinalong with Bulk and Marketed Formulation. Research Journal of Pharmacy and Technology. 2026;19(4):1618-4. doi: 10.52711/0974-360X.2026.00231 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-4-20
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