ABSTRACT:
Actinomycetes are a diverse group of heterotrophic prokaryotes forming hyphae at some stage of their growth hence referred to as filamentous prokaryotes. This group is a potential producer of many enzymes, enzyme inhibitors, growth promoting substances and antibiotics. Endophytic actinomycetes have been defined as that can be isolated from the disinfected surfaces of plant tissues or that can be extracted from within the plant that do not cause visible harm to the host. They can promote the growth of many field crops by producing plant growth-promoting substances and potential sources of novel natural products for exploitation in medicine, agriculture and industry. It is noteworthy that, of the nearly 300,000 plant species that exist on the earth, In our study we have isolated the endophytic actinomycetes from the different parts of the citrus plants. The total 7 actinomycetes were isolated from different parts of citrus plant of different species (Citrus aurantifolia) using Starch Casein agar and YMA media, out of 7 actinomycetes strains, 3 actinomycetes showing antibacterial activity were recovered using Bennet agar media. Two from Citrus aurantifolia twig using Starch Casein agar and one actinomycetes was isolated from Citrus aurantifolia twig using YMA media. The production of antibacterial compound was performed using L.B. Broth. Out of 3 actinomycetes strain only one show (CT1 isolated from Citrus aurantifolia twig) the strong antibacterial activity and fermentation carried out using L.B. Broth for 15 days. After fermentation, extraction of the supernatant was carried out using solvent petroleum ether. The Antibacterial compound was recovered using TLC and Column Chromatography. The Rf value of the compound was found to be (0.61403). The structural study of the extracted compound was carried out by UV-spectroscopy, FT-IR and NMR. The antibacterial compound was effective against E. coli, S. typhi, K. pneumoniae, S. aureus bacteria some of them got resistance against some antibiotic drug.