ABSTRACT:
The aim of present study was the isolation, purification, partial characterization and antibacterial activities of compound produced by some actinomycetes from sedimented waters. Actinomycetes are gram positive, non motile, non capsulated bacteria that are characterized by the formation of branching filaments. The emergence of pathogenic bacteria which are resistant to multiple antibiotics, represent growing threat to human health and has given additional importance to the search for the newer drugs. Approximately two-thirds of the known secondary metabolites are produced by members of the order actinomycetales. The water samples of the region of Tamilnadu, Uttar Pradesh, and Madhya Pradesh have the capability to produce Actinomycetes. Luria-Bertani agar medium was one of the best medium for the isolation of Actinomycetes from sedimented water. The antibacterial assay of isolated Actinomycetes was carried out against some gram positive and gram negative microorganisms. The cultural characteristics of the isolated strains were studied from the antibacterial assay and the strain ST6 was selected for further study. Fermentation of ST6 was carried out by using synthetic medium 1. The recovery of the fermentation broth was carried by vaccum filtration and centrifugation. Extract of supernatants S1, S2 were carried out by using solvent ethyl acetate, chloroform. The two extracts SAM 1, SAM 11 were recovered. Their antibacterial activity was studied by using E.coli (ATCC 11880), S.aureus (ATCC 29737), S. typhi (ATCC 2356), M.luteus (ATCC 11880). The zone of inhibition was observed. Sam 11 showed good zone of inhibition as compared to SAM 1. The analysis of the two extracts were carried out by the Rf values were found to be as 0.8 (SAM 1), 0.79 (SAM 11). Also from UV and IR studies the SAM 1 and SAM 11 showed the ?max of 230nm and 250nm. The IR spectrum of SAM 1 indicated the functional groups like C=O, C-O, NH, aromatic substituted compound and for SAM 11 indicated the functional groups like C=O, C-O, NH, HX and aromatic substituted compound. The DNA of ST6 was extracted by cell lysis technique and purification was carried out by agarose gel electrophoresis. The DNA bands were separated according to the molecular weight as compared with standard marker DNA.