ABSTRACT:
Two novels, simple, rapid and sensitive spectrophotometric method has been developed for simultaneous estimation of Nimesulide and dicyclomine HCl. The first method involved determination of Nimesulide and Dicyclomine HCl using the Q analysis method and second method involved simultaneous equation method. Nimesulide has absorbance maxima at 300.8 nm, Dicyclomine HCl has absorbance maxima at 217.6 nm and isoabsorptive point is at 232.4 nm in methanol. Linearity was obtained in the concentration ranges 10-100 µg/ml and 900-3000 µg/ml for Nimesulide and Dicyclomine HCl, respectively. These methods were successively applied to pharmaceutical formulation because no interferences from tablet excipients were found. Accuracy and reproducibility of proposed method was statistically validated by recovery studies. The suitability of these methods for the quantitative determination of the compounds was proved by validation.