ABSTRACT:
The free radicals are having much importance in the body and which they lead to cell degradation via different path ways. To avoid the free radical interaction with the cells in the body the spin trapping agents normally nitraso compounds are used to trap them later which they leads to formation of Spin adduct easily excreted by the body. In This current research the methyl and ethyl radicals are trapped by using 3-Carboxy-PROXYL and 3-Carbamoyl-PROXYL and were finally identified by Gas Chromatography Mass Spectrometry. Initially, Fenton chemistry along with the 3-Carboxy-PROXYL and/or 3-Carbamoyl-PROXYL is used for the generation of hydroxyl radicals, and then these are used in the oxidation of di-methyl sulfoxide (DMSO), and ethanol producing methyl and ethyl radicals that were spin trapped and were identified by GC/MS. This research was indirectly used to detect hydroxyl radicals by trapping methyl and ethyl radicals.