Author(s):
T. Nageswara Rao1*, G. Silpa2 and T. Srinivasarao3
Email(s):
Email ID Not Available
DOI:
Not Available
Address:
1Department of Engg Chemistry, Andhra University, Visakhapatnam, AP, India
2Department of Biotechnology, Indian Institute of Technology (IIT), Madras, TamilNadu, India
3Johnson& Johnson-Health Care Products & Pharmaceuticals, Mumbai, Maharashtra, India.
Published In:
Volume - 5,
Issue - 10,
Year - 2012
ABSTRACT:
The aim of the present work was to develop and validate a liquid chromatographic method for the determination of memantine hydrochloride in tablet dosage form. Chromatographic separation was achieved on a intersil ODS 3v column (150×4.6 mm), 5 µm column with a HPLC grade methanol and 0.1% trifluroacetic acid buffer in the ratio of 50:50 (v/v/v) as mobile phase under ELS Detector conditions like a evaporation temperature 700 C, nebulization temperature 400 C, nitrogen flow rate 1.2SLM, and LED intensity 90%, injection volume is 20µL, flow rate is 1.0 mL/min and the expected retention of memantine hydrochloride peak is about 6.8 minutes. Response was a linear function of concentration in the range 2-0.01 µg/mL for memantine hydrochloride; correlation coefficiencent was 0.9998, respectively. LOD and LOQ for memantine hydrochloride were found 0.01 µg/mL and 0.03 µg/mL based on signal to noise ratio(S/N). Accuracy (recoveries 88-96%) and reproducibility were found to satisfactory. The present method can be applied successfully for the quantification of active pharmaceutical ingredient content in the combined formulations of memantine hydrochloride.
Cite this article:
Not Available
Cite(Electronic):
Not Available Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2012-5-10-5