Dileep N., Rakesh K.N., Syed Junaid, Poornima G., Swarnalatha S.P., Prashith Kekuda T.R.
Dileep N.1,2, Rakesh K.N.1,2, Syed Junaid1,2, Poornima G.1,2*, Swarnalatha S.P.1, Prashith Kekuda T.R.1
1Department of Microbiology, 2Department of Biochemistry, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India.
Volume - 5,
Issue - 10,
Year - 2012
The present study examined the antioxidant activities of different solvent extracts of the ripe pericarp of Polyalthia longifolia Thw. (Annonaceae).The powdered ripe pericarp was extracted successively with ethyl acetate, chloroform and ethanol. Antioxidant activity of solvent extracts was determined by DPPH free radical scavenging assay and Ferric reducing assay. Total phenolic content was estimated by Folin-Ciocalteau method. The solvent extracts scavenged DPPH free radical dose dependently with IC50 value of 52.22±0.05, 61.82±0.01 and 06.85±0.05µg/ml for ethyl acetate, chloroform and ethanol extract respectively. An increase in the absorbance with increase in the concentration of extracts was observed in ferric reducing assay indicating reducing potential. The phenolic content of ethyl acetate, chloroform and ethanol extract was found to be 95±0.09, 85±0.01 and 224±0.10 µg Gallic acid equivalents/mg of extract respectively. Ethanol extract showed potent antioxidant activity than other extracts and this effect could be attributed to high phenolic content in ethanol extract. To our knowledge, this is the first report on antioxidant activity of solvent extracts of ripe pericarp of P. longifolia.
Cite this article:
Dileep N., Rakesh K.N., Syed Junaid, Poornima G., Swarnalatha S.P., Prashith Kekuda T.R. In vitro Antioxidant Activity of Ripe Pericarp of Polyalthia longifolia Thw. Research J. Pharm. and Tech. 5(10): October 2012; Page 1312-1315.
Dileep N., Rakesh K.N., Syed Junaid, Poornima G., Swarnalatha S.P., Prashith Kekuda T.R. In vitro Antioxidant Activity of Ripe Pericarp of Polyalthia longifolia Thw. Research J. Pharm. and Tech. 5(10): October 2012; Page 1312-1315. Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2012-5-10-6
1. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Springer Verlag, Berlin, 2007, 341.
2. Murthy MM, Subramanyam M, Bindu HM and Annapurna J. Antimicrobial activity of clerodanediterpenoids from Polyalthia longifolia seeds. Fitoterapia76(3-4); 2005: 336-339
3. Faizi S, Khan RA, Mughal NR, Malik MS, Sajjadi KE and Ahmad A. Antimicrobial activity of various parts of Polyalthia longifolia var. pendula: Isolation of active principles from the leaves and the berries. Phytotherapy Research 22(7); 2008: 907-912
4. Bose S, Byahatti VV, D’Souza M and Bose A. Antioxidant and antimicrobial activities of isolated constituents from the bark of Polyalthia longifolia. International Journal of Green Pharmacy 4(2); 2010: 93-97
5. Manjula SN, Kenganora M, Parihar VK, Kumar S, Nayak PG, Kumar N, Pai RKS and Rao CM. Antitumor and antioxidant activity of Polyalthia longifolia stem bark ethanol extract. Pharm Biol 48(6); 2010: 690-696
6. Malairajan P, Gopalakrishnan G, Narasimhan S and Veni KJK. Evaluation of anti-ulcer activity of Polyalthia longifolia (Sonn.)Thwaites in experimental animals. Indian Journal of Pharmacology 40(3); 2008: 126-128
7. Pal D, Bhattacharya S, Baidya P, De BK, Pandey JN and Biswas M. Antileishmanial activity of Polyalthia longifolia leaf extract on the in vitro growth of Leishmaniadonovani promastigotes. Global Journal of Pharmacology 5(2); 2011: 97-100
8. Saleem R, Ahmed M, Ahmed SI, Azeem M, Khan RA, Rasool N, Saleem H, Noor F and Faizi S. Hypotensive activity and toxicology of constituents from root bark of Polyalthia longifolia var. pendula. Phytotherapy Research 19(10); 2005: 881-884
9. Ghosh G, Kar DM, Subudhi BB and Mishra SK. Anti-hyperglycemic and antioxidant activity of stem bark of Polyalthia longifolia var. angustifolia. Der Pharmacia Lettre 2(2); 2010: 206-216
10. Tanna A, Nair R and Chanda S. Assessment of anti-inflammatory and hepatoprotective potency of Polyalthia longifolia var. pendula leaf in Wister albino rats. Journal of Natural Medicines 63(1); 2009: 80-85
11. Sharma RK, Mandal S, Rajani GP, Gupta N, Srivastava DP. Antiulcer and antiinflammatory activity of fresh leave extracts of Polyalthia longifolia in Rats. International Journal of Drug Development and Research 3(1); 2011: 351-359
12. Sivashanmugam AT and Chatterjee TK. Anticataractogenesis activity of Polyalthia longifolia leaves extracts against glucose-induced cataractogenesis using goat lenses in vitro. European Journal of Experimental Biology 2(1); 2012: 105-113
13. Sugumaran M, Bharathi V and Lakshmi HRM. Ethnomedicinal Plants for Indigestion in Uthiramerur Taluk, Kancheepuram District, Tamil Nadu, India. Journal of Chemical and Pharmaceutical Research 2(6); 2010: 463-470
14. Kekuda TRP, Raghavendra HL, Swathi D, Venugopal TM and Vinayaka KS. Antifungal and Cytotoxic Activity of Everniastrum cirrhatum (Fr.) Hale. Chiang Mai Journal of Science. 39(1); 2012: 76-83
15. Kekuda TRP, Vinayaka KS, Swathi D, Suchitha Y, Venugopal TM and Mallikarjun N. Mineral Composition, Total Phenol Content and Antioxidant Activity of a Macrolichen Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae). E-Journal of Chemistry 8(4); 2011: 1886-1894
16. Devasagayam TPA, Baloor KK and Mishra KP. Some new methods for free radical research. SFRR-India Bulletin 2(2); 2003: 20-28
17. Dixit P, Ghaskadbi S, Mohan H and Devasagayam TPA. Antioxidant properties of germinated fenugreek seeds. Phytotherapy Research 19; 2005: 977-983
18. Chatterjee S, Poduval BT, Tilak JC and Devasagayam TPA. A modified, economic, sensitive method for measuring total antioxidant capacities of human plasma and natural compounds using Indian saffron (Crocus sativus).Clinica Chimica Acta 352; 2005: 155-163
19. Shirwaikar A, Prabhu KS and Punitha ISR. In vitro antioxidant studies of Sphaeranthus indicus (Linn). Indian Journal of Experimental Biology 44; 2006: 993-996
20. Blois MS. Antioxidant determinations by the use of stable free radical. Nature 29; 1958: 1199
21. Di Mambro V M, Azzolini A E C S, Valim Y M L and Fonseca J V. Comparison of antioxidant activities of tocopherols alone and in pharmaceutical formulations. International Journal of Pharmaceutics 262; 2003: 93–99
22. Lim YY, Lim TT and Tee JJ. Antioxidant properties of several tropical fruits: A comparative study. Food Chemistry 103; 2007: 1003-1008
23. Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in Oxidative Defense systems of various senescing Leaves. Journal of Agricultural and Food Chemistry 43; 1995:1813-1817
24. Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. Journal of Agricultural and Food Chemistry 50; 2002: 2454-2458
25. Tilak JC, Adhikari S and Devasagayam TPA. Antioxidant properties of Plumbago zeylanica, an Indian medicinal plant and its active ingredient, plumbagin. Redox Report 9(4); 2004: 220-227
26. Coruh N, Celep AGS, Ozgokce F and Iscan M. Antioxidant capacities of Gundeliatournefortii L. extracts and inhibition on glutathione-S-transferase activity. Food Chemistry 100; 2007: 1249-1253
27. Behera BC, Verma N, Sonone A, Makhija U. Determination of antioxidative potential of lichen Usneaghattensisin vitro. LWT 39; 2006: 80-85
28. Rekha C, Poornima G, Manasa M, Abhipsa V, Devi PJ, Kumar VHT and Kekuda TRP. Ascorbic acid, Total phenol content and antioxidant activity of fresh juices of four ripe and unripe citrus fruits. Chemical Science Transactions 1(2); 2012: 303-310.