ABSTRACT:
A high fat diet increased the body weight and produce adiposity in humans. Pancreatic lipase plays a major role in digestion and absorption of dietary fat because the dietary fat is not directly absorbed from intestine. So it is possible to reduce the absorption of dietary fat by the inhibition of pancreatic lipase, thereby obesity can be managed. Orlistat is one of the clinically approved drugs for treatment of obesity in the mechanism of pancreatic lipase inhibition. The aqueous ethanolic extract (70%) of Dalbergia sissoo Roxb. leaves (DSEE) and orlistat as reference has been studied for potent pancreatic lipase inhibition using chicken pancreas (Gallus domesticus) in dose dependent manner. The preliminary phytochemical analysis of extract showed the presence of flavonoids, glycosides, tannins, and terpenoids. The pancreatic lipase inhibition (IC50) value for the orlistat and extract was found to be 2.049±0.98 and 3.89±0.97 which might be attributed to the presence of phytoconstituents. Hence DSEE can be used as an anti-obesity agent in suitable form.