ABSTRACT:
Simple, precise and economical spectrophotometric method have been developed for the simultaneous estimation of Metoprolol Succinate (METO) and Amlodipine(AMLO) in combined tablet dosage form. Present study describes the development and validation for the simultaneous estimation of metoprolol(METO) and Amlodipine(AMLO) by the Ratio derivative UV spectroscopy method. Methanol is used as solvent. The amplitudes at 277.017nm and 235.62nm of the first derivative of ratio spectra were selected to determine METO and AMLO, respectively by ratio derivative method. Beer’s law is obeyed in the concentration range of 50-250 µg/mL and 5-25 µg/mL for Metoprolol Succinate and Amlodipine, respectively by both the methods. The % assay in commercial formulation was found to be 99.56% for METO and 98.96% for AMLO by the proposed method. The method was validated with respect to linearity, precision and accuracy. Recovery was found in the range of 99.51-99.92% for METO and 99.69-100.6% for AMLO by ratio derivative method. The present result shows that the proposed method can be successfully employed for routine analysis of the marketed formulations.