Author(s):
Thorat Shashikant D., Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R.
Email(s):
thorat_shashikant@ymail.com
DOI:
Not Available
Address:
Thorat Shashikant D.*, Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R.
Department Pharmaceutical Chemistry, Amrutvahini Collage of Pharmacy, Sangamner, Dist- Ahmednagar, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 6,
Issue - 10,
Year - 2013
ABSTRACT:
A simple, specific, accurate and precise Reverse Phase High Performance Liquid Chromatography method was developed for simultaneous estimation of Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in powder dosage form on RP C-18 Column (EC 250/4 neucleosil 100-10) using Methanol : Water (97:3 %v/v) as mobile phase. The flow rate was 1.0 ml/min and effluent was monitored at 274nm. The retention time for Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) was found to be as 8.78 and 12.80 respectively. Proposed method was validated for Specificity, Precision, Accuracy, Linearity, and Robustness.
Cite this article:
Thorat Shashikant D., Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cholecalcererol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in Powder Dosage Form. Research J. Pharm. and Tech. 6(10): October 2013; Page 1107-1110.
Cite(Electronic):
Thorat Shashikant D., Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cholecalcererol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in Powder Dosage Form. Research J. Pharm. and Tech. 6(10): October 2013; Page 1107-1110. Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2013-6-10-19