ABSTRACT:
Cleistanthus collinus leaf extracts were prepared in various organic solvents and were subjected to study the phytochemical profile using Gas Chromatography - Mass Spectroscopy method. The aqueous, methanol, ethanol and ethyl acetate hot extracts of sample was prepared from shade dried plant leaf powder by using Soxhlet apparatus. The chemical compositions of the plant leaf extracts of C. collinus were investigated using Gas Chromatography - Mass Spectroscopy (Agilent-7890A GC instrument coupled with MS-5975) and NIST-MS library. GC-MS analysis of C. collinus plant leaf extracts revealed the existence of the GC-MS chromatogram of the major peaks presented in water extract (4-O-Methylmannose - 70.12%), ethyl acetate extract (Silane, trimethyl [5-methyl-2-(1-methyl ethyl) phenoxy]- Anthracene 7.06%), ethanol extract (4-C-methyl- (Methylthio)-acetonitrile 4-O-Methylmannose - 58.53%) and methanol extract (Thiophene, tetrahydro-2-methyl- Myo-Inositol - 48.54%). From this study it is obvious that C. collinus leaf extracts contains many biologically active compounds in various concentrations and also it gives a detailed insight about the phytochemical profile which could be exploited for the development of plant based drug.