ABSTRACT:
This study scientifically supports the usage of plant as a remedy for various superficial bacterial and fungal infections in traditional medicine. The findings of the current study also can be useful to progress further scientific investigation on the leaf of this species. The present study deals with preliminary phytochemical analysis and antimicrobial activity of in vitro condition Asparagus racemosus Willd. leaf with various extracts like petroleum ether, methanol, chloroform, acetone, ethyl acetate and water. The presence and absence of the primary and secondary phytoconstituents like Alkaloids, Glycosides, Cardiac Glycosides, Saponins, Tannins and Phenolic Compounds, Flavonoids, Anthroquinone, Proteins and Amino Acids, Sterols and Triterpenoids and Carbohydrates were detected by usual prescribed methods. The effect of different extracts were tested on Gram positive bacteria like Bacillus subtilis, Staphylococcus aureus and Gram negative bacteria E coli, Pseudomonas aeruginosa and the yeast Candida utilis by agar well diffusion method. In the present study showed the presence of primary and secondary metabolites like alkaloids, tannins, phenolic compound, Flavonoids, steroids, Saponins, glycosides and carbohydrates and proteins in the Methanolic extracts than other extracts like chloroform, Acetone, Petroleum ether, Ethyl acetate and Water. The maximum antimicrobial activity at 100µg/ml was found for Staphylococcus aureus (20mm) and Escherichia coli (20mm) in methanol and minimum 8 mm in Escherichia coli in petroleum ether leaf extracts of A.racemosus In vitro condition and no activity of fungi was observed against some extracts.