ABSTRACT:
Objective: To evaluate the membrane stabilizing potential and inhibition of protein denaturation property of hydroalcoholic extract Phyllanthus fraternus Webster [PF].
Method – Hydroalcoholic extract of PF was evaluated using the human red blood cells (HRBC) membrane stabilization method as HRBC membranes are similar to lysosomal membrane components. The prevention of hypotonicity-induced HRBC membrane lysis was taken as a measure of anti-inflammatory activity of PF extract. While the extract was also incubated with egg albumin and subjected to determination of absorbance and viscosity to assess the anti-inflammatory property.
Results- The hydroalcoholic extract of PF in concentration of 25-800 microgram/ml showed a concentration dependent inhibition of haemolysis of erythrocytes induced by hypotonic solution. The hydroalcoholic extract of PF also showed a concentration dependent inhibition of protein (albumin) denaturation with IC50 of 52 microgramml.
Conclusion- From the study it can be postulated that the anti-inflammatory activity of Phyllanthus fraternus Webster could be due to its membrane stabilizing action and inhibition of protein denaturation.
Cite this article:
Atul Ramchandra Chopade, Fahim Jehangir Sayyad. Evaluation of membrane stabilizing and inhibition of protein denaturation activity of Phyllanthus fraternus Webster. Research J. Pharm. and Tech. 6(3): March 2013; Page 251-254.
Cite(Electronic):
Atul Ramchandra Chopade, Fahim Jehangir Sayyad. Evaluation of membrane stabilizing and inhibition of protein denaturation activity of Phyllanthus fraternus Webster. Research J. Pharm. and Tech. 6(3): March 2013; Page 251-254. Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2013-6-3-17