ABSTRACT:
The plant Givotia rottleriformis have been used traditionally for treating skin diseases like psoriasis, for which there is no complete cure till date. Psoriasis, is a genetically determined chronic inflammatory dermatosis immuno disorder associated with over expression of proinflammatory cytokines characterized by red, scaly and raised patches that affects 2.3% of the population worldwide. According to American academy of dermatology, antibacterial therapy could also be used for treating psoriasis. In view of this, the present investigation was carried out to study the antibacterial activity with respect to their traditional use as antipsoriatic agents. The powdered bark was successively extracted with ethyl acetate and methanol and subjected to preliminary phytochemical analysis and HPTLC finger print analysis. The antimicrobial studies of ethyl acetate and methanol extract was carried out by agar diffusion method. Among the ethyl acetate and methanol extract, methanol extract exhibited highest zone of inhibition at the concentration of 1000 ppm against Staphyloccocus aureus (18 mm) which has been one of the organisms which aggravate conditions like psoriasis, while moderate activity against Streptococcus pyogenes (12 mm). The zone of inhibition of ethyl acetate extract against Escherichia coli was 11 mm and against Streptococcus pyogenes was 14 mm at higher concentration (1000 ppm). The methanolic extract was ineffective against the fungal strain Candida albicans and ethyl acetate extract exhibited moderate effect (11 mm) against fungal strain at higher concentration (1000ppm). The minimum inhibitory concentration was also determined and found that the methanolic extract was required in relatively lesser quantities for arresting the growth of the tested organisms. The results obtained may provide validation for its reported medicinal uses.