ABSTRACT:
Cyclooxygenase–2 (COX–2) inhibition is an important approach in the management of inflammatory problems. Since synthetic compounds may exaggerate the levels of inflammatory mediators via COX pathway, so many researchers are focusing in search of natural compound or bioguided fraction for COX–2 inhibitors. From the literature review it is evident that natural polyphenols such as flavonoid and tannins have been found exert its anti-inflammatory effects either by decreasing formation of reactive oxygen species or by modulation of pro-inflammatory gene expression of Cyclooxygenase by acting through Nuclear factor – kappa B (NF-kappa) and Mitogen Activated Protein Kinase signaling. Earlier literature revealed that Terminalia chebula (Fruits); Sida rhombifolia (Root) Leucas cephalotes (Entire plant) were found to contain polyphenolic compounds and steroid and are used in Indian traditional system of medicine for inflammatory associated problems. The earlier scientific reports showed the extract of the above plants have significant anti-inflammatory activity in animal model. Hence the present study is focused to separate the fraction from the selected plants and study the effect of combined fractions for inhibition of inflammatory key enzymes .Further the bioactive fraction was standardized by HPTLC as per standard protocol. The outcome of the present work would pave a pathway for finding a new phytopharmacophore from Indian meditational plants for treatment of inflammatory associated diseases.