ABSTRACT:
Primary congenital glaucoma (PCG) is an aggressive type of glaucoma occurs early in life. Cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1) enzyme, which is considered as phase I xenobiotic metabolizing enzyme, controls the eye oxidative homeostasis and correspondingly the normal development of the eye. The concentrations of CYP1B1 enzyme, and total antioxidant capacity and malondialdehyde as parameters reflecting the oxidative stress were determined using enzyme-linked immunosorbent assay and spectrophotometric methods. The study sample was included 100 patients diagnosed with primary congenital glaucoma and other 100 healthy subjects served as controls. The age of all the patients and the controls ranged between one month and three years. CYP1B1enzyme concentration was higher in PCG patients than in controls (0.867±0.11 ng/ml versus 0.524±0.06 ng/ml, p=0.01), malondialdehyde was also higher in PCG patients than in controls (0.968±0.06 µmol/L versus 0.359±0.02 µmol/L, p=0.0001), while the levels of total antioxidant capacity were lower in PCG patients (2.790±0.48 U/ml for patients versus 7.031±0.65 U/ml for controls, p=0.0001). Our findings indicate that high oxidative stress is related to PCG disease. Oxidative stress is one of the factors involved in the pathogenesis of primary congenital glaucoma and high CYP1B1enzyme concentrations are associated with high oxidative stress.