ABSTRACT:
To evaluate the Insilico docking study of the compounds identified from the leaves of Orthosiphon stamieus for Hepatoprotective activity. In the present study, in-silico docking analysis was carried out for the compounds present in leaves of Orthosiphon stamineus in comparison with standard Silymarin against Pregnene X receptor and NF-?b Receptor. Experiments were performed using the program GLIDE (Grid-based Ligand Docking with Energetic) module version 5.9, Schrödinger, LLC, New York, NY, 2013 (Schrodinger Inc.). Compounds 1,2 and 4 have shown significant Pregnene X receptor inhibitory activity. Of the 3 compounds, compounds 1 and 4 showed maximum Pregnene X receptor inhibition than compound 2 and standard drug Silymarin. Compounds 1,4 and 7 have shown significant NF-?b Receptor inhibitory activity. Of the 3 compounds , compounds 1 and 4 showed maximum NF-?b Receptor inhibition than the compound 2 and the standard drug Silymarin. The study showed that the leaves of Orthosiphon stamineus is potential for the hepatoprotective activity by inhibiting Pregnene X receptor( PXR) and Nuclear Factor kappalight- chain-enhancer of activated b cells ( NF-?b Receptor)