ABSTRACT:
Since various and numerous pathogenic microorganisms can infect and dwell human's urogenital tract, and because the ability of bacteria to develop resistance to antibiotics, in addition to the side effects of some of these antibiotics in treatment of infectious diseases, therefore we need to alternative herbal and non-antibiotic therapy for treating the urogenital tract infections such as Garlic (Allium sativum L.). The aim of the study is to investigate the antimicrobial effects of Garlic extract on local uropathogenic species of Escherichia coli, Proteus mirabilis and Trichomonas vaginalis in cases of urogenital tract infections. The tested urogenital microorganisms were isolated and identified according to diagnostic features as golden of morphology and biophysiology for Escherichia coli, Proteus mirabilis and Trichomonas vaginalis. Fresh garlic bulbs were obtained from local markets of Iraq. The aqueous garlic extract (AGE) was performed according to standard method. The antibacterial activity of AGE was done against motility, adherence and biofilm formation, and compared with some UTI antibiotics. The antiparasitic activity of AGE was done against growth rate, motility and viability of cultured vaginalis in vitro by use medium. current study suggest that the AGE has antibacterial effects against E. coli and Pr. mirabilis. The diameter of the inhibition zone was ranged from 27.0 to 28.0 mm for garlic extract, as compared with 12.0 to 18.0 mm for Ciprofloxacin against both genera; E. coli and Proteus mirabilis, respectively. The efficacy of adherence and biofilm formation by E. coli and Pr. Mirabilis was much reduced. The motility of these bacteria was completely inhibited following AGE exposure to 30%, and temporally inhibited by 20%. Furthermore, the growth rate, viability and motility of the studied protozoan parasite " vaginalis" were completely inhibited following exposure to crude extract of AGE post 1 hour, completely inhibited following AGE exposure of 30% post 24 hour, completely inhibited by 20% post 48 hour, completely inhibited by 10% post 72 hour and temporally inhibited by 5% post 96 hour, respectively.