Author(s):
Jung-Eun Park, Jong-Hwa Jang
Email(s):
Je3207@naver.com , jhjang@hanseo.ac.kr
DOI:
10.5958/0974-360X.2018.00338.4 
Address:
Jung-Eun Park1, Jong-Hwa Jang*2
1Department of Preventive and Social Dentistry, School of Dentistry, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, Republic of Korea
2Department of Dental Hygiene, Hanseo University, 360 Daegok-Ri, Haemi-Myun, Seosan-Si, Chungnam,
356-706, Republic of Korea
*Corresponding Author
Published In:
Volume - 11,
Issue - 5,
Year - 2018
ABSTRACT:
Background/Objectives: The purpose of this research was to determine a method to analyze several salivary anions simultaneously using HPLC (IC) and to analyze the anions in actual saliva samples. Methods/Statistical analysis: Chromatographic determination was performed using a Dionex Ion column Pac AG 11-HC 50mm×4mm. The column parameters were as follows: mobile phase, isocratic elution with 120 mmol/L NaOH; flow rate, 1,000 µL/min; separation temperature, 35°C. Findings: The linearity of the sample’s linear range was 0.9902-0.9989, LOD was 0.78-1.16µM, and LOQ was 2.36-3.49 µM. The recovery test showed a recovery rate of 95.63-107.77%, and RSD of 3.37-11.48%, verifying the method of analysis. Improvements/Applications: The method of analysis used in this study can appropriately quantify anions in saliva. Hence, it could be used in biomarker studies.
Cite this article:
Jung-Eun Park, Jong-Hwa Jang. Development and Application of an Analytical Method for Salivary Inorganic Anion Quantification for the Diagnosis of Systemic and Oral Diseases. Research J. Pharm. and Tech 2018; 11(5):1820-1824. doi: 10.5958/0974-360X.2018.00338.4
Cite(Electronic):
Jung-Eun Park, Jong-Hwa Jang. Development and Application of an Analytical Method for Salivary Inorganic Anion Quantification for the Diagnosis of Systemic and Oral Diseases. Research J. Pharm. and Tech 2018; 11(5):1820-1824. doi: 10.5958/0974-360X.2018.00338.4 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2018-11-5-23