ABSTRACT:
Pots experiment was conducted during the summer season 2016 in the Department of Biology/College of Education/AL– Qadisiya University to study the effect of nano chelated iron, GA3 and organic fertilizer (Acadian) in the Content of a-tocopherol and Phytosterols (gamma-Sitosterol, Stigmasterol and Campesterol) in Moringa leaves. The experiment was designed as Randomized Complete Blocks (RCBD) in a factorial arrangement with three replicate. These are involved 5 concentrations of nano chelated (0, 1, 2, 3 and 4) g.l-1, GA3 concentrations were used (0, 200 and 400) mg.l-1 and organic fertilizer Acadian with (0,1) g.l-1. After 150 days from the date of germination, Using the GCMS device to measure the percentage a-tocopherol and Phytosterols. Means were compared by using least significant difference (LSD) at 0.05 level probability. Results showed: the concentration of 2g.L-1 of the nano-chelated iron achieved the highest percentage of Stigmasterol and Campesterol. 4 g.L-1of the nano-chelated ironsignificant decrease in the percentageof a-tocopherol. No significant effect of nano-chelated iron on the percentage of gamma-Sitosterol was found. The concentration of 200 mg.l-1 GA3 significantly increased plant sterols (gamma-Sitosterol, Stigmasterol and Campesterol). GA3 had a negative effect on the percentage of a-tocopherol, Acadian had no significant effect on the active substances studied.The three-way interaction of the studied factors showed that the highest percentage of a-tocopherol increased with the use of 2 g.l-1nano-iron with 200 mg. l-1GA3 and 1 g.l-1of Acadian fertilizer. The use of 1 g.l-1nano-iron with 400 mg. l-1GA3 and 1 g.l-1of Acadian fertilizer to increase the percentage of Stigmasterol, while the combination of 1g.l-1nano-iron with 200 mg. l-1GA3 and 1 g.l-1of Acadian increased the percentage of Campesterol Therefore, we recommend using the combinations above to obtain the highest percentage of active substancesa-tocopherol, Stigmasterol and Campesterol due to its economic feasibility.