ABSTRACT:
Thalassemia is the most common congenital hemolytic anemiadue to partial or complete lack of synthesis of _-globin chains caused by mutations that affect the synthesis this-chains. The aim of this study is the detection of IL-10-1082A\G polymorphism and IL-10 serum level with it correlation to pathogeneses of disease. Case-control study was performed of 60 patients with ß-TM diagnosed at thalassemia-center in AL-Zahra hospital in AL-Najaf city, Iraq with group of 40 healthy individual was used as control, the patients were ( 26 male and 34 female) at age3–49 and about 18 patients infected with virus.Blood sample and serum was collected from all patients and control. Blood used for DNA extracted for using SSP-PCR in detection the IL-10-1082A\G polymorphism. IL-10 level were measured by enzyme-linked immunosorbent assay test. The result shown that male (56.6%) more than female (42.6%), and the age range (10-19) were highest than other age range. This result explain that HCV infected patients less than non-infected thalassemia patients and the infected male more than female, the age group (10-19) was more infected with HCV. The result demonstrate that AA genotype and A allele is risk factor of severity in thalassemia patients, while GG genotype and G allele is protective factor for severity. The result explain that GG genotype is risk factor for HCV infection. This result also shown that IL-10 level is significantly increase in thalassemia patients than control, also significantly increase in IL-10 level in thalassemia with HCV infection than other patients with no HCV infection and control. Conclusion: The polymorphism in IL-10 at position (1082A\G) has association with pathogeneses of thalassemia at AA genotype, and IL-10 consider as predictive factor for severity of thalassemia