Author(s): Razan Zohairee, Mohammad Amer Zamrik, Sophie Barguil

Email(s): rita.joo@hotmail.com

DOI: 10.5958/0974-360X.2018.00618.2   

Address: Razan Zohairee1, Mohammad Amer Zamrik2, Sophie Barguil3
1Master Student DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University.
2Professor DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University.
3Assistant Professor DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University.
*Corresponding Author

Published In:   Volume - 11,      Issue - 8,     Year - 2018


ABSTRACT:
Background: Benzo [a] pyrene is considered a classic DNA-damaging carcinogen and is a lead compound of polycyclic aromatic hydrocarbons.B [a] Pis metabolically activated by cytochrome P450 enzymes to formation electrophilic metabolites such as epoxides. These epoxides might bind to DNA and produce DNA-adducts. ELISA method is used for determination of DNA adducts of benzo [a] pyrenediolepoxide (BPDE). Objectives: The aim of this study was to evaluate exposure to B [a] P in asphalt plant workers by measuring the BPDE-DNA adducts in their peripheral white blood cells (WBC), which are considered biological markers for exposure risk assessment. MaterialandMethods: In this study, the levels of BPDE-DNA adducts were measured in DNA samples of WBC obtained from asphalt plant workers in Syria and compared to those measured from a control group. The measurement was performed using BPDE-DNA Adducts ELISA kit. The sample size was determined to be 50 with 25 asphalt plant workers and 25 healthy volunteers with no occupational exposure to Benzo [a] pyrene. Results: BPDE-DNA adducts were detected in WBC of 11 asphalt plant workers with concentrations ranging between 0 and 2.75 ng/ml and only one individual in the control group with concentration of 0.75 ng/ml. These results indicate a significant positive relationship betweenexposure toB [a] P through the bitumen fumes (working in asphalt plant) and formation of BPDE-DNA adducts. Conclusions: BPDE-DNA adducts area potential biomarker for PAHs exposure and likely helpful indicator of PAH-induced DNA damage.


Cite this article:
Razan Zohairee, Mohammad Amer Zamrik, Sophie Barguil. Detection of Benzo[a] pyrenediol epoxide-DNA adducts in White Blood Cells of Asphalt Plant Workers in Syria. Research J. Pharm. and Tech 2018; 11(8): 3363-3366. doi: 10.5958/0974-360X.2018.00618.2

Cite(Electronic):
Razan Zohairee, Mohammad Amer Zamrik, Sophie Barguil. Detection of Benzo[a] pyrenediol epoxide-DNA adducts in White Blood Cells of Asphalt Plant Workers in Syria. Research J. Pharm. and Tech 2018; 11(8): 3363-3366. doi: 10.5958/0974-360X.2018.00618.2   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2018-11-8-23


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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