ABSTRACT:
Summary: One of the most essential roles in polymerase chain reaction (PCR) primer design is a gene structure, predominantly for eukaryotic cells genes with numeral single-nucleotide polymorphisms (SNPs) which impose on users the discrimination between them via many techniques involving PCR. At this point, PRIMER1 is illustrated as suitable network server used for primer design to detect SNP by a simple and economical method named tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR). In PRIMER1, the users are able to concentrate their attention on the dbSNP NCBI and primer design delineation without wasting time identifying the positions of SNPs manually configuring difficult limitation. Accessibility: PRIMER1 is freely accessible at: http://primer1.soton.ac.uk/primer1.html. The network server supports the browsers: Firefox, Chrome, Safari, Opera, and Torch.
Cite this article:
Dhafer A. F. Al-Koofee, Shaden M. H. Mobarak. PRIMER1: A Network Service for Tetra-Arms PCR Primer Design Based on Well-Known dbSNP. Research J. Pharm. and Tech 2018; 11(8): 3633-3637. doi: 10.5958/0974-360X.2018.00669.8
Cite(Electronic):
Dhafer A. F. Al-Koofee, Shaden M. H. Mobarak. PRIMER1: A Network Service for Tetra-Arms PCR Primer Design Based on Well-Known dbSNP. Research J. Pharm. and Tech 2018; 11(8): 3633-3637. doi: 10.5958/0974-360X.2018.00669.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2018-11-8-74