Author(s): Rajan V. Rele, Sandip P. Patil

Email(s): drvinraj@gmail.com

DOI: 10.5958/0974-360X.2019.00829.1   

Address: Rajan V. Rele*, Sandip P. Patil
Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai 400016
*Corresponding Author

Published In:   Volume - 12,      Issue - 10,     Year - 2019


ABSTRACT:
A novel reverse phase liquid chromatographic method was developed and validated for estimation of anti-protozoal, anti-amebic and antigiardiasis drug, secnidazole in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Peerless basic C18 (50mm x 4.6mm, 3µm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 80:20 % (v/v). The flow rate was maintained at 1 ml / min. The detection was carried out at wavelength 310 nm. The method was validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50-150 µg/ml for secnidazole. The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination secnidazole of in dosage form with good recoveries.


Cite this article:
Rajan V. Rele, Sandip P. Patil. Development of Analytical method by RP-HPLC Technique for determination of Secnidazole in API and Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 2019; 12(10):4799-4802. doi: 10.5958/0974-360X.2019.00829.1

Cite(Electronic):
Rajan V. Rele, Sandip P. Patil. Development of Analytical method by RP-HPLC Technique for determination of Secnidazole in API and Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 2019; 12(10):4799-4802. doi: 10.5958/0974-360X.2019.00829.1   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2019-12-10-39


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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