ABSTRACT:
Ezogabine is an Anti-convulsant drug. A simple, accurate and reproducible high performance thin layer chromatographic (HPTLC) method for the determination of ezogabine has been developed and validated as per ICH guidelines. The HPTLC separation was achieved on the aluminium plates precoated with silica gel 60F254 using Toluene: Ethyl acetate: Formic acid (3:6:1 v/v/v) as mobile phase. Quantification was achieved with HPTLC detection at 254nm over the concentration range of 1-6µg/band with recovery in the range of 98.15- 100 % for Ezogabine. The correlation coefficient of ezogabine was found to be 0.999. The RSD values of ezogabine for interday and intraday precision was found to be 0.17- 0.28% and 0.16-0.21% respectively. LOD and LOQ values for ezogabine were found to be 0.102µg/spot and 0.309µg/spot respectively. Retention factor (Rf) for Ezogabine was 0.67. The method showed acceptable values for accuracy, precision and robustness studies. The proposed method was simple and can be used for routine analysis of ezogabine in laboratories.