Author(s):
Meyyanathan. S. N, Babu. B, Kalaivani. M
Email(s):
snmeyyanathan@jssuni.edu.in
DOI:
10.5958/0974-360X.2019.00354.8 
Address:
Lingamallu Venkata Sai Krishna1, Narenderan. S. T1, Ramshankar Nayak1,
Meyyanathan. S. N*1, Babu. B1, Kalaivani. M2
1Department of Pharmaceutical Analysis, JSS College of Pharmacy (JSS Academy of Higher Education and Research, Mysuru) Udhagamandalam, India.
2Indian Pharmacopoeia Commission, New Delhi.
*Corresponding Author
Published In:
Volume - 12,
Issue - 5,
Year - 2019
ABSTRACT:
A rapid LC-MS/MS method has been developed and validated for the quantitative determination of Febuxostat from the commercially available formulations. The separation was achieved using Zorbax SB C18 column (4.6 × 50 mm, 5 µm) as a stationary phase and the mobile phase consists of (10 mM) Ammonium formate (pH4.0): methanol (10:90 v/v) with a flow of 0.6 mL/min. Detection was carried out by triple quadrupole mass spectrometry with electrospray ionization in positive mode with proton adducts at m/z 316.95?260.95 to monitor Febuxostat within a run time of 2 min. The linearity of the method was found over a concentration range of 4 to 55 ng/mL with a regression analysis of 0.997. The percentage recovery of the present method was found to be 97.16 to 99.46%. The LC-MS/MS method was validated as per ICH guidelines. The developed method can be successfully applied for the estimation of Febuxostat in the commercial formulation and in bulk drug.
Cite this article:
Meyyanathan. S. N, Babu. B, Kalaivani. M. Development and Validation of An Analytical Liquid Chromatography-Tandem Mass Spectroscopy Method for the Estimation Febuxostat in Pharmaceutical Formulation. Research J. Pharm. and Tech. 2019; 12(5):2137-2140. doi: 10.5958/0974-360X.2019.00354.8
Cite(Electronic):
Meyyanathan. S. N, Babu. B, Kalaivani. M. Development and Validation of An Analytical Liquid Chromatography-Tandem Mass Spectroscopy Method for the Estimation Febuxostat in Pharmaceutical Formulation. Research J. Pharm. and Tech. 2019; 12(5):2137-2140. doi: 10.5958/0974-360X.2019.00354.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2019-12-5-13