ABSTRACT:
Newcastle diseases (ND) it is viral disease infected birds and it is infectious, highly contagious diseases and cause respiratory and digestive distress with neurological signs and sudden death combined with high mortality and morbidity may arrived to (93%) in field infection. In Iraq, poultry industry suffered from high mortality arrived to (90%) or more with severe respiratory distress with greenish diarrhea, neurological signs and sudden death, grossly characterized by tracheitis, with hemorrhage of Payer?s patches, cecal tonsils and proventriculus that lead to high economic losses in poultry industry, so that the aim of this study were isolated NDV with identification by using HA-HI test and RT-PCR assays with specific designing primer. A total of 65 samples (lung, treache, spleen and intestine) were collected from organized farms in karbala city, suspected infected with NDV and after processing these samples were inoculated in to the allantoic cavity of (9-11) days old embryonated eggs with cultivation for five days then collected allantoic fluid and stored in deep freeze. The HA-HI test was conducted to identify NDV by using private antisera, twenty one samples from sixty five are infected with NDV. The molecular methods (RT-PCR) test also confirmed twenty one samples infected with NDV, Nucleotide sequences of NDV was obtained from NCBI websites and alignment with other researchers concerned in this topic.