ABSTRACT:
Background: Asthma is currently understood to be a fairly common but complicated chronic respiratory disease with multiple etiologies. Polymorphism in the CysLTR2 gene resulting in a single amino acid substitution, M201V (i.e. amino acid methionine changed for valine at the 201 position of CysLTR2 protein). Objectives: The aims of this study included analyzing of polymorphism in CyLTR2 M01 V genes and determined whether there is an interaction and association between these polymorphisms and some parameter (total IgE, IL-5, Eosinophil) for asthma development in Iraqi asthmatic patients. Methods: 100 patients with asthma with ages ranged between (15- 50) years were taken from (Al-Hussein Medical City/Kerbala). Control group consisted of 60 healthy people who were free from signs and symptoms of Asthma who matched in age and gender with patients, and had no history for any asthma problem. Total IgE and IL-5 Euroimmun /Ggermen, Peprotech UK respectively) was studied using the enzyme-linked immunosorbent assay (ELISA) method and automated blood cell counter (Sysmex XT-200i)for eosinophil counts, the CyLTR2 M01 V also studied by using RFLP PCR . T-test and ANOVA and Pearson correlation used to analyze results by using SPSS version 20. P-value = 0.05 was considered significant. Results: Total IgE, IL-5 levels and Eosinophil counts were increased significantly (p< 0.05) in patients compared with control group. Also there were show significant abnormality and complication when compared with control groups, there Regarding to CyLTR2 M01 V gene polymorphism were done by RFLP PCR there is no statistical difference between control and asthmatic patients. also there are a statistically significant difference in serum IgE . serum IL-5 and blood eosinophil between each two groups depending on polymorphism in cysteine leukotriene receptor 2 (CyLTR2 M01 V). Conclusion: there are a significantly correlation between total IgE, IL-5 levels and eosinophil counts and polymorphism in cysteine leukotriene receptor 2 (CyLTR2 M01 V)in asthmatic patients . but CysLTR1 927A/T gene polymorphism no show statistical difference between control and asthmatic patients.